Value of plasma microRNAs has-let-7d and has-let-7e as potential molecular markers in workers with occupational exposure to mercury
10.3760/cma.j.issn.1001-9391.2017.10.003
- VernacularTitle: 血浆微RNA hsa-let-7d和hsa-let-7e作为职业性汞作业工人潜在分子标志物的研究
- Author:
Jun GUO
1
;
Hongqun ZHANG
;
Enmin DING
;
Ying BAI
;
Baoli ZHU
Author Information
1. Yandu District Center for Disease Control and Prevention, Yancheng 224002, China
- Publication Type:Journal Article
- Keywords:
microRNA;
Plasma;
Biomarker;
Mercury
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(10):732-736
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether plasma microRNAs has-let-7d and has-let-7e can be used as potential molecular markers for workers with occupational exposure to mercury.
Methods:October 2013, the workers with occupational exposure to mercury who worked in a mercury thermometer factory and participated in occupational health examination were selected as subjects, and biological samples and basic data were collected. The subjects were divided into chronic mercury poisoning group,mercury absorption group,and control group,with 30 individuals in each group. AmicroRNA microarray combined with RT-qPCR was used to measure the expression of has-let-7d and has-let-7e in the three groups, the receiver operating characteristic(ROC)curve was used to analyze the values of has-let-7d and has-let-7e in the diagnosis of occupational chronic mercury poisoning,a software platform was used to predict target genes. Gene ontology enrichment analysis and KEGG pathway analysis were also performed.
Results:Compared with the control group, the chronic mercury poisoning group and the mercury absorption group had significant increases in the expression of has-let-7d and has-let-7e(P<0.05). The areas under the ROC curve for microRNAs has-let-7d and has-let-7e in the diagnosis of occupational chronic mercury poisoning were 0.912(95% confidence interval[CI]0.843-0.981)and 0.908(95%CI0.837-0.979),respectively,and there was a significant difference between them(P<0.001).
Conclusion:Plasma microRNAs has-let-7d and has-let-7e can be used as potential molecular markers for workers with occupational exposure to mercury.
