Correlation analysis of the Epstein-Barr virus recruited regulatory T cells in nasopharyngeal carcinoma of immune escape
10.3760/cma.j.issn.1673-0860.2017.09.011
- VernacularTitle: 鼻咽癌免疫逃逸中EB病毒募集Treg细胞相关性分析
- Author:
Pei BI
1
;
Jie WANG
1
;
Juan LU
1
;
Xiong LIU
1
;
Fan WANG
1
;
Yunfan LUO
1
;
Shudao PENG
2
;
Xiangping LI
1
Author Information
1. Department of Otorhinolaryngology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
2. Department of Health Management Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal neoplasms;
T-lymphocytes, regulatory;
Epstein-Barr virus;
Immune escape
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2017;52(9):692-697
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relation of Epstein-Barr virus (EBV)-induced immune escape to regulatory T cells(Treg) in nasopharyngeal carcinoma(NPC).
Methods:We recruited 83 patients who were pathologically diagnozed as nasopharyngeal carcinoma and treated in Nanfang Hospital from January to September 2016. CD4+ CD25+ FOXP3+ Treg in peripheral blood was examined by flow cytometry and the level of EBV DNA in the blood was detected by quantitative real-time polymerase chain reaction(qRT-PCR)in 83 patients and 51 healthy adults as control. The expression of FOXP3+ Treg and EBER in NPC in 32 of 83 patients and in nasopharyngitis in 16 control patients were tested by immunohistochemistry(IHC) and in-situ hybridization(ISH).
Results:The ratio of Treg in blood in patients with NPC(5.29±1.45)% was higher than that in control group (4.78±1.19) %, with a statistically significant difference(P=0.035). The ratio of Treg in blood in patients with positive EBV was higher than that in patients with negative EBV(P=0.013), and there was a positive correlation between EBV DNA copies and Treg ratio(r=0.335, P=0.002). The density of FOXP3+ Treg in NPC was higher than that in nasopharyngitis(P=0.001). Positive correlation was found between the density of FOXP3+ Treg and the stain intensity of EBER(r=0.496, P=0.004).
Conclusion:The ratios of Treg in blood and tissue in NPC patients with positive EBV are significantly increased, indicating a role of Treg in EBV-induced immune escape in NPC.
