Expression of large tumor suppressor homolog 2 gene and its promotor methylation in oral squamous cell carcinoma
10.3760/cma.j.issn.1002-0098.2017.09.012
- VernacularTitle: 口腔鳞状细胞癌中大肿瘤抑制因子2基因的表达及启动子甲基化水平分析
- Author:
Zengwen YUE
1
;
Jinzhong LIU
1
Author Information
1. Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
- Publication Type:Journal Article
- Keywords:
Carcinoma, squamous;
Genes, tumor suppressor;
Blotting, western;
DNA methylation;
Large tumor suppressor homolog 2
- From:
Chinese Journal of Stomatology
2017;52(9):569-573
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of large tumor suppressor homolog 2 (LATS2) gene and its promotor methylation in oral squamous cell carcinoma (OSCC).
Methods:Reverse transcription-PCR (RT-PCR) and pyrosequencing were used to detect the mRNA and promotor methylation of LATS2 gene in 72 OSCC specimens and normal oral mucosa tissues. Western blotting was used to detect the LATS2 protein in six OSCC specimens and normal oral mucosa tissues.
Results:All cases had expression of LATS2 mRNA in normal oral mucosa tissues, but the expression was down-regulated significantly, only 47% (34/72) in 72 cases of OSCC showed LATS2 mRNA expression. The expression was correlated with the degree of tumor differentiation and lymph node metastasis (P<0.05). The results of pyrosequencing show that 68% of promotor methylation (49/72) in 72 cases of OSCC. Furthermore, there was significant correlation between the mRNA and promotor methylation of LATS2 gene (χ2=16.980, P<0.01). All the six specimens had the low LATS2 protein expression.
Conclusions:The promotor methylation of LATS2 gene may play an important role in the occurrence of OSCC.
