Study on the correlation of the effect of entecavir on Th1/Th2 cytokines level in the treatment of chronic hepatitis
10.3760/cma.j.issn.1007-3418.2017.08.010
- VernacularTitle: 恩替卡韦对慢性乙型肝炎患者外周血Th1/Th2型细胞因子表达水平的影响
- Author:
Ling LI
1
;
Yangbin JING
;
Jiao LIU
2
;
Chuanlin WANG
2
;
Bing LIU
1
,
3
Author Information
1. Sichuan Mianyang 404 Hospital, Mianyang 621000, China
2. Department of Infectious Diseases, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China
3. Department of Nephrology, Yanting People's Hospital of Sichuan Mianyang, Yanting Mianyang 621600, China
- Publication Type:Journal Article
- Keywords:
Hepatitis B, chronic;
Cytokines;
Th1 cells;
Th2 cells;
Entecavir
- From:
Chinese Journal of Hepatology
2017;25(8):606-611
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression level of peripheral blood Th1/Th2 type cytokines of chronic hepatitis b (CHB) patients in the entecavir (ETV) antiviral treatment, analyze the relationship between various cytokines, and the correlation among of cytokines and HBV DNA loads.
Methods:Luminex Liquid Chip Technology was applied to detect the peripheral blood Th1/Th2 type cytokines expression level of CHB patients; At the same time, liver function was detected by Fully Automatic Biochemical Analyzer; HBV DNA loads were detected by PCR Method; Hepatitis b virology markers were detected by Chemiluminescence Method. F-test and Pearson correlation analysis were used for statistical analysis.
Results:Before ETV antiviral treatment, peripheral blood Th1 cytokines IFN gamma expression level in patients with CHB increased significantly (P = 0.010) compared with the healthy control group while TNF alpha expression level having no statistically significant difference (P = 0.095); Th2 type cytokines IL-4, IL-6, IL-10 levels decreased obviously (P = 0.039, P = 0.014, P = 0.026) compared with those in the control group. After 48 weeks of treatment, Th1 cytokines IFN gamma and TNF alpha expression levels were reduced significantly (19.2±5.03 pg/ml vs 24.69±6.51 pg/ml, and 6.09±4.99 pg/ml vs 9.50±7.34 pg/ml, P < 0.001, and P = 0.016) compared with that before treatment; Th2 type cytokines IL-4, IL-6 expression level increased significantly (33.86±22.47 pg/ml vs 21.32±12.84 pg/ml, and 11.65±6.91 pg/ml vs 8.51±4.94 pg/ml, P = 0.004, and P = 0.029) compared with that before treatment while IL-10 expression level having no statistical significance (P = 0.081). There was disorder and irregularity in the correlation between the levels of Th1/Th2 type cytokines. And there was no correlation between the various cytokines and HBV DNA loads in patients with CHB.
Conclusion:ETV can not only inhibit HBV DNA replication, reducing HBV DNA loads, but also contribute to regulate Th1/Th2 type cytokines expression level in patients with CHB, but there was no correlation between the levels of various cytokines, various cytokines and HBV DNA loads.
