Effect of dentin proteoglycans on the stability of resin-dentin bonds against artificial saliva storage
10.3760/cma.j.issn.1002-0098.2017.08.008
- VernacularTitle: 牙本质内源性聚糖对树脂-牙本质粘接界面耐水储存老化能力的影响
- Author:
Yu GAO
1
;
Sanjun ZHAO
2
;
Peihuan WANG
3
;
Shuai LU
4
;
Jihua CHEN
2
Author Information
1. Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University& State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Xi'an 710032, China(Present address: Department of Pediatric Dentistry, School of Stomatology, Shandong University & Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China)
2. Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University& State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Xi'an 710032, China
3. Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University& State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Xi'an 710032, China(Present address: Department of Stomatology, The General Hospital of Jinan Military Command, Jinan 250031, China)
4. Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University& State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Xi'an 710032, China(Present address: Department of Stomatology, The Official Hospital of Chengdu Military Command, Chengdu 610011, China)
- Publication Type:Journal Article
- Keywords:
Proteoglycans;
Dentin-bonding agents;
Tensile strength;
Nanoleakage
- From:
Chinese Journal of Stomatology
2017;52(8):486-491
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the potential effect of proteoglycans (PG) and glycosaminoglycans (GAG) on the stability of resin-dentin bonds against artificial saliva storage.
Methods:Seventy-two extracted molars were used to obtain standard dentin bonding surface, and the specimens were etched for 15 s with 37% phosphoric acid and divided into three groups using a table of random number. Then the three groups undergone different incubating procedures as follow: specimens in chondroitinase ABC (C-ABC) group were incubated with C-ABC, specimens in trypsin (TRY) group were incubated with trypsin, and specimens in the control group were incubated with deionized water. All specimens were incubated at 37 ℃ for 48 h in the oscillators. Then specimens in each group were randomly assigned into three subgroups (n=8) as follows: immediate control subgroup, aging subgroups with artificial saliva storage for 6 months and 12 months. Microtensile bond strength (μTBS), fracture mode, bonding interface morphology and nanoleakage were evaluated.
Results:Immediately and with artificial saliva storage for 6 months and 12 mouths, the μTBS of TRY group ([49.04±3.57], [37.01±3.21] and [35.27±3.56] MPa) were significantly higher than those in the control group ([40.71±3.32], [28.87±2.34] and [24.20±2.07] MPa) (P<0.05). The immediate μTBS of C-ABC group ([32.94±2.45] MPa) was significantly lower than that of the control group (P<0.05). While with artificial saliva storage for 6 months and 12 mouths, the μTBS of C-ABC group ([26.46±2.45] and [22.50±2.58] MPa) were no differences with those of the control group (P>0.05). The ratio of cohesive fracture increased with the extension of aging time. Some narrow gaps were found in hybrid layer of the control group with artificial saliva storage for 6 months and 12 mouths.
Conclusions:Removal of PG increased the μTBS and durable bonds to dentin, while removal of GAG decreased the μTBS, however, it can be of help to create more durable bonds to dentin.
