Determination of fluorine-ion in human urine by ion chromatography method
10.3760/cma.j.issn.1001-9391.2017.08.019
- VernacularTitle: 人尿中氟测定的离子色谱法
- Author:
Fugang ZHANG
1
;
Huifang YAN
;
Yajuan PAN
;
Wei ZHAO
Author Information
1. National Institute of Occupational Health and Poison Control, China CDC, Beijing 100050, China
- Publication Type:Journal Article
- Keywords:
Ions;
Chromatography;
Fluorine;
Urine
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(8):622-624
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method to determine fluorine ion in human urine by ion chromatography method.
Methods:The samples were centrifuged, diluted and processed by C18 solid-phase column, potassium hydroxide as mobile phase, gradient elution, separated by AS15, and determined by anion conductivity detector.
Results:The linear correlation coefficient of fluoride ion in the range of 0.02-10.00 mg/L was more than 0.999. The lowest detection limit of urinary fluoride was 0.06 mg/L (in 3 ml urine) . The recoveries of the method were 96.9%-99.5%, the inter batch precision range was 0.40%-2.89%. The intra batch precision was from 1.01 to 7.23%. Fluoride samples could be stored for 7 days at room temperature and 4 ℃, and be stored for 14 days at -20 ℃.
Conclusion:Determination of fluoride in urine by ion chromatography, the method could meet the requirement in GBZ/T 210.5-2008.
