Detection of HLA-B*13:01 gene by dual allele-specific real-time polymerase chain reaction in patients with trichlorethylene-induced dermatitis
10.3760/cma.j.issn.1001-9391.2017.08.008
- VernacularTitle: 双重等位基因特异性实时聚合酶链反应检测三氯乙烯药疹样皮炎患者HLA-B*13:01基因
- Author:
Dafeng LIN
1
;
Yanhua YANG
;
Dianpeng WANG
;
Peimao LI
;
Zhimin ZHANG
;
Yanfang ZHANG
;
Xianqing HUANG
Author Information
1. Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen 518020, China
- Publication Type:Case Reports
- Keywords:
Trichloroethylene;
Medicamentosa-like dermatitis;
Polymerase chain reaction;
HLA-B*13:01
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(8):589-591
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the detection of a human leukocyte antigen-B (HLA-B) allele HLA-B*13:01 by dual allele-specific real-time polymerase chain reaction (PCR) in patients with trichlorethylene-induced dermatitis.
Methods:A total of 20 patients with trichlorethylene-induced dermatitis who were admitted and treated from January 2014 to October 2016 were enrolled as case group, and 20 persons who underwent physical examination from January to October, 2016 were enrolled as control group. Peripheral cubital venous blood samples were collected from all subjects, and dual allele-specific real-time PCR was used to detect the HLA-B*13:01 gene. The two groups were compared in terms of the proportion of subjects carrying HLA-B*13:01 gene.
Results:There were no significant differences between the case group and the control group in median age (25.0 years vs 27.0 years, Z=0.30, P>0.05) and the proportion of male subjects (60.0% vs 70.0%, χ2=0.44, P>0.05) . The mean time of exposure to trichloroethylene was 30.8 days in the case group, while the subjects in the control group were not exposed to trichloroethylene. The case group had a significantly higher frequency of HLA-B*13:01 gene than the control group (80.0% vs 20.0%, χ2=14.40, P<0.01) with an odds ratio of 16.00.
Conclusion:Dual allele-specific real-time PCR can be used for detection of the HLA-B*13:01 gene in patients with trichlorethylene-induced dermatitis.
