Survey of HIV-1 drug resistance threshold in Shandong Province in 2013-2015
10.3760/cma.j.issn.0253-9624.2017.07.006
- VernacularTitle: 山东省2013—2015年新发现未治疗HIV-1感染者耐药毒株传播警戒线调查
- Author:
Xiaoguang SUN
1
;
Haiying YU
;
Shengli SU
;
Bin LIN
;
Jinhai LI
;
Lin LIN
;
Xiaorun TAO
;
Yuesheng QIAN
;
Dianmin KANG
;
Hui XING
Author Information
1. Shandong Center for Disease Control and Prevention, Jinan 250014, China
- Publication Type:Journal Article
- Keywords:
HIV;
Sequence analysis;
Antiretroviral therapy;
Drug resistance threshold survey
- From:
Chinese Journal of Preventive Medicine
2017;51(7):604-609
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To survey the prevalence of drug resistant HIV-1 in Shandong province in 2013-2015.
Methods:WHO truncated sequential sampling technique was adopted by using 77 and 53 samples of newly diagnosed as HIV-1 positive and aged 16-25 years in Shandong province in 2013 and 2015. RNA was prepared and HIV-1 pol region was amplified by RT-PCR and nested PCR. Pol genetic mutation associated with drug resistance was analyzed.
Results:The success rates for sequence acquisition of the survey were 100% (77/77) and 94% (50/53) in 2013 and 2015, and the main subtype was CRF01_AE. A total of 2 surveillance drug-resistance mutation(SDRMs) and 3 SDRMs were found by analyzing the 47 sequences each year, sampled in 2013 and 2015, indicating that the prevalence of drug resistant HIV-1 stains was low in 2013, and moderate in 2015. A total of 5 individuals with drug resistant HIV-1 stains found in this study were mainly infected by homosexual transmission (3 cases), and the other two samples were different: one was infected by heterosexual transmission, the other was infected by IDU. The subtype was CRF01_AE (2 cases) , CRF07_BC (2 cases) and B (1 case) . SDRMs for protease inhibitor (PIs), nucleotide HIV-reverse transcriptase inhibitor (NRTIs) and non-NRTI (NNRTIs) were all found in the individuals with drug resistant HIV-1 stains.
Conclusion:CRF01_AE were the main HIV-1 subtypes of recently reported HIV-infected individuals in Shandong province, and the HIV-1 drug resistant strains transmission was catalogued as at low and moderate prevalence level in 2013 and 2015.
