Antagonism of lovastatin on oxidative stress and apoptosis in primary rat hippocampal neurons induced by β-amyloid peptide
10.3760/cma.j.issn.0529-5807.2017.07.009
- VernacularTitle: 洛伐他汀对β-淀粉样肽诱导原代大鼠海马神经细胞氧化应激及凋亡的拮抗作用
- Author:
Longchun TAN
1
;
Liang ZHAO
;
Xianhong LIU
;
Chengmin DENG
;
Zhizhong GUAN
Author Information
1. The Key Laboratory of Molecular Biology, Guizhou Medical University, Guiyang 550004, China
- Publication Type:Journal Article
- Keywords:
Lovastatin;
Amyloid beta-peptides;
Free radicals;
Apoptosis;
Neurons;
Cells, cultured
- From:
Chinese Journal of Pathology
2017;46(7):491-496
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of lovastatin on oxidative stress and apoptosis in neurons induced by β-amyloid peptide (Aβ).
Methods:Primary culture of rat hippocampal neuron was treated with Aβ oligomers alone or combined with lovastatin. The levels of OH-, H2O2, O2·-, malondialdehyde, superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activities were measured by biochemical methods and protein expression of caspase-3 and bcl-2 was detected by Western blot.
Results:As compared with the control group, treatment of 0.5 μmol/L Aβ oligomers for 48 h led to significant increase of OH-, H2O2, O2·- and malondialdehyde content, inhibition of SOD and GSH-PX activities, enhanced caspase-3 expression and decreased bcl-2 expression. Interestingly, these neurotoxic modifications on the levels of OH-, H2O2, O2·- and malondialdehyde content, SOD and GSH-PX activities, and the protein expression of cleaved caspase-3 and bcl-2 were significantly attenuated when the cells were pretreated with 0.1 μmol/L lovastatin for 24 h before exposure of Aβ oligomers.
Conclusion:Lovastatin may play an important role in antagonizing the neurotoxicity of Aβ through a mechanism likely related to the inhibition of oxidative stress.
