Effects of Wnt3a on osteogenic differentiation of dental pulp stem cells
10.3760/cma.j.issn.1002-0098.2017.07.007
- VernacularTitle: Wnt3a蛋白对牙髓干细胞成骨向分化的影响
- Author:
Yanyan SUN
1
;
Weiping HU
2
;
Zongxiang LIU
3
;
Wei WANG
1
Author Information
1. Department of Prosthodontics, Xuzhou Stomatology Hospital, Xuzhou Jiangsu 221000, China
2. Department of Prosthodontics, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China
3. Department of Periodontics, Xuzhou Stomatology Hospital, Xuzhou Jiangsu 221000, China
- Publication Type:Journal Article
- Keywords:
Wnt proteins;
Dental pulp stem cells;
Osteogenesis
- From:
Chinese Journal of Stomatology
2017;52(7):427-431
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Wnt3a on osteogenic differentiation of human dental pulp stem cells (DPSC).
Methods:DPSCs were subjected to different concentrations of Wnt3a (0, 5, 20, 50 and 100 μg/L) and at seven days after culture the alkaline phosphatase (ALP) activity was tested. Mineralized nodule formation was examined by alizarin red staining. Osteogenic-related gene expression of bone sialoprotein (BSP), osteocalcin (OCN), collagen type Ⅰ (COL-Ⅰ), Runt-related transcription factor-2 (RUNX2) was examined by quantitative real-time PCR (qPCR).
Results:After seven days of induction by DPSC, Wnt3a protein could inhibit the ALP activity (concentration 0: 1.076±0.203, 5 μg/L: 0.828±0.118, 20 μg/L: 0.505±0.044, 50 μg/L: 0.499±0.038, 100 μg/L: 0.483±0.060). The expression of OCN in 5 μg/L Wnt3a group (0.092±0.005) was lower than that in culture medium (0.858±0.190)(P<0.05). Alizarin red staining showed that 5 μg/L Wnt3a had no mineralization induction effect on DPSC.
Conclusions:Wnt3a could inhibit osteogenic differentiation of dental pulp stem cells.
