Application of immunohistochemistry for p16 and GATA3 and molecular HPV typing in diagnosis of secondary bladder involvement by cervical carcinoma
10.3760/cma.j.issn.0529-5807.2017.06.005
- VernacularTitle: p16、GATA3表达及人乳头状瘤病毒分型检测在宫颈癌累犯膀胱病理诊断中的价值
- Author:
Rongfang HUANG
1
;
Cheng HE
;
Weifeng ZHU
;
Yan LI
;
Gang CHEN
Author Information
1. Department of Pathology, Fujian Cancer Hospital, Fujian Medical University Cancer Hospital, Fuzhou 350014, China
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
Cyclin-dependent kinase inhibitor 16;
GATA3 transcription factor;
Human papillomavirus 16;
Immunohistochemistry
- From:
Chinese Journal of Pathology
2017;46(6):388-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of p16 and GATA3 and the detection of human papillomavirus (HPV) in secondary bladder involvement by cervical carcinomas.
Methods:Sixteen cases of cervical carcinoma with bladder involvement diagnosed from December 2008 to March 2016 were collected and evaluated by light microscopy, immunohistochemistry for p16 and GATA3 detection and PCR-reverse dot blot for molecular typing of HPV.
Results:The age of the patients ranged from 25 to 76 years with median of 52 years. Morphologically, 14 cases(14/16) showed tumor nests infiltrating lamina propria or muscle bundles of the bladder. By immunohistochemistry, 15 cases (15/16) were found to be diffusely and strongly positive for p16, and 1 showed patchy staining pattern. Seven cases (7/7) of corresponding original cervical cancers were also diffusely and strongly positive for p16. GATA3 staining was negative in 13 cases (13/16), and focal weak to moderate positivity was detected in 3 cases.Three cases (3/7) of corresponding original cervical cancers showed focal weak to moderate positivity of GATA3. Fifteen cases (15/16) showed concordant high risk HPV-positivity, including HPV16 in 8 cases and HPV31 in one case. Five cases showed co-infection of HPV16 and HPV18. One case showed co-infection with HPV18 and HPV45.
Conclusion:Differential diagnosis by p16 or GATA3 alone is of limited value. Combination of immunohistochemistry for p16 and GATA3 and molecular typing for HPV detection are useful to distinguish primary bladder carcinoma from the secondary involvement by cervical carcinoma.
