Survey on anti-Pneumocystis jirovecii recombinant antigen IgG and IgM antibodies in children under 14 years of age
10.3760/cma.j.issn.1003-9279.2017.06.013
- VernacularTitle: 14岁以下儿童抗耶氏肺孢子菌合成抗原IgG和IgM抗体的调查
- Author:
Shanna WU
1
;
Shuang GUO
2
;
Qing’e JIN
1
;
Xiaojun TIAN
2
;
Wei LI
2
;
Shaogang LI
2
;
Yi REN
2
;
Gang WANG
1
Author Information
1. Clinical Laboratory Department, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
2. Beijing Tropical Medicine Research Institute, Beijing Key Laboratory for Research on Prevention and Treament of Tropical Disease, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
- Publication Type:Journal Article
- Keywords:
Pneumocystis jirovecii;
Recombinant major surface glycoprotein;
Antibody
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(6):541-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain recombinant major surface glycoprotein(rMsg)of Pneumocystis jirovecii(Pj) and survey anti-Pj rMsg IgG and IgM antibodies using enzyme-linked immunosorbent assay (ELISA) among randomly selected sera from Beijing local children under 14 years of age.
Methods:ELISA method were established to detect anti-Pj rMsg IgG and IgM antibodies. We randomly selected sera of 201 cases who were under 14 years of age were screened.
Results:There were 78 cases in the age group of 1 day to 30 days, 33 cases in the group of 1 month to 12 months, and 90 cases in the group of 1 year to 14 years. The positive rates of anti-Pj rMsg IgG antibody in these three age groups were 10.3%(8/78), 21.2%(7/33), and 41.1%(37/90)respectively(χ2=21.190, P=0.000). The positive rates of anti-Pj rMsg IgM antibody were 6.4%(5/78), 39.4%(13/33), and 76.7%(69/90)respectively(χ2=84.260, P=0.000). The overall positive rates of anti-Pj rMsg IgG and IgM antibodies were 25.9%(52/201)and 43.3%(87/201)respectively(χ2=53.463, P=0.000). Among these, simultaneously IgG positive IgM positive, IgG positive IgM negative, IgG negative IgM positive and IgG negative IgM negative rates were 22.4%(45/201), 3.5%(7/201), 20.9%(42/201), and 53.2%(107/201)respectively.
Conclusions:The overall serological response rate after Pj exposure, either anti-Pj IgG or IgM antibody positive, was as high as 46.8% in children under 14 years of age in Beijing area. The positive rates of anti-rMsg IgG and IgM antibodies increased with age, with highest rate in age group of more than one years old.
