Molecular epidemiological characteristics of Human Calicivirus in Kunming city, 2014-2015
10.3760/cma.j.issn.1003-9279.2017.06.009
- VernacularTitle: 2014-2015年昆明市杯状病毒分子流行特征研究
- Author:
Liying HE
1
,
2
;
Yongming ZHOU
3
;
Wenpeng GU
3
;
Wanfu XU
4
;
Shunxian ZHANG
4
,
5
;
Lin AI
6
;
Yan LU
6
;
Liguang TIAN
6
Author Information
1. The First Affiliated Hospital of Jinan University, Guangzhou 510630, China
2. Department of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou 510623, China
3. Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China
4. Department of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou 510623, China
5. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China
6. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China
- Publication Type:Journal Article
- Keywords:
Human Calicivirus;
Norovirus;
Diarrhea;
Children
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(6):525-529
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the molecular epidemiological characteristics of Human Calicivirus (HuCV) infection among children less than 5 years in Kunming city, Yunnan province, it might be provide effective evident for prevention and control the diarrhea related with HuCV infection.
Methods:Four sentinel hospitals were recruited in the study from Kunming city, Yunnan province, 850 diarrhea cases and 170 non-diarrhea subject were recruited in this study from 2014 to 2015. RT-PCR was performed to screen HuCV infection, and gene sequencing was used to ensure positive infection subtypes and genotypes.
Results:The positive rate of HuCV was higher in children with diarrhea than in non-diarrhea children (11.5%, 98/85; 4.7%, 8/170, χ2=7.083, P=0.008), and the positive rate of Norovirus (NoV) GII was higher in non-diarrhea children were (11.1%, 94/85; 4.7%, 8/170, χ2=6.353, P=0.012). There was no significant difference in the positive rate of NoV GI (0.1%, 1/850; 0.0%, 0/170, P=0.833) and Sappovirus (0.4%, 3/850; 0.0%, 0/170, P=0.578) in diarrhea children and non-diarrhea children. GII.P4 (10%, n=102) was the most important genotype of NoV GII detected in diarrhea and non-diarrhea individuals. Despite no significant difference in Norovirus GII infection between different age groups (χ2=0.038, P=0.846) and sex(χ2=0.620, P=0.733), infection rate of NoV GII varied with season (χ2=9.867, P=0.020), having close relationship with diarrhea in autumn (15.6%), primarily caused by GII.4 and GII.12 genotype (χ2=8.881, P=0.031; χ2=7.917, P=0.039).
Conclusions:NoV GII diarrhea had higher epidemic rate, which was caused by multiple genotypes, GII.P4 was a dominant genotype, and was a major pathogenic agent of diarrhea in infants f in Kunming city.
