Molecular genotyping of measles viruses in Yancheng area in 2016 and analysis of the genetic characterization of hemagglutinin genes
10.3760/cma.j.issn.1003-9279.2017.06.006
- VernacularTitle: 2016年盐城地区麻疹病毒分子分型及血凝素蛋白基因特征分析
- Author:
Guoqing CHEN
1
;
Chunxiang LI
2
;
Yao WANG
1
;
Feng LI
1
;
Shilin XU
1
;
Changcheng LI
1
;
Rongbiao SHAO
1
Author Information
1. Yancheng Center for Disease Control and Prevention, Yancheng 224001, China
2. Yancheng Maternity and Child Health Care Hospital, Yancheng 224002, China
- Publication Type:Journal Article
- Keywords:
Measles virus;
Genotype;
HA gene;
N gene;
Sequence analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(6):509-514
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the genotypes and the genetic evolution of the hemagglutinin genes of measles viruses in the city of Yancheng in 2016.
Methods:Using a set of primers and probes for screening positive for measles viruses, specimens of throat swab were detected using the method of real time RT-PCR. The sequences of the nucleoprotein and hemagglutinin genes of measles viruses were amplified through one step RT-PCR method and the PCR products were sequenced. The sequences of nucleotide and amino acid of the nucleoprotein and hemagglutinin genes of measles viruses were analyzed and the evolutional trees were generated using bioinformatics software.
Results:The genotypes of measles viruses in the Yancheng area in 2016 included subgenotype H1a and genotype D8. Phylogenetic trees analysis showed that the five representative strains of subgenotype H1a in Yancheng area and Jiangxi representative strain (KJ136545) clustered into independent evolutionary branches, belonged to the clade of H1a -1 evolutionary genes. The seven representative strains of genotype D8 in Yancheng area were clustered with the American representative strain in 2009 (JN635404), belonged to the D8-3-2 small clade genes. Compared with vaccine strain of Shanghai S191, the amino acid site in 240thof the five representative strains of subgenotype H1a in Yancheng area mutated from serine to asparagine, leading to a loss of the N-glycosylation site NLS238-240. The seven representative strains of genotype D8 in Yancheng area had no change in N-glycosylation.
Conclusions:In 2016, the prevalent strains of measles viruses in Yancheng area were mainly Chinese H1a dominant subgenotype and D8 imported genotype. In addition to a loss of the N-glycosylation site NLS238-240in 240thof the five representative strains of subgenotype H1a, most of the major neutralizing antigen sites of hemagglutinin gene of measles viruses in Yancheng area did not mutate. The Chinese vaccine of Shanghai S191 can effectively prevent infection caused by subgenotype H1a and subgenotype D8 strains.
