Plasmacytoid dendritic cells and CD4+ T helper cells function in acute and chronic hepatitis B patients
- VernacularTitle: 急、慢性乙型肝炎患者浆细胞样树突状细胞与辅助性CD4+T细胞的功能研究
- Author:
Weihua CAO
1
;
Chongping RAN
2
;
Tianlin QI
2
;
Xingyue WANG
2
;
Xiaojing QU
2
;
Dan ZHANG
2
;
Lu ZHANG
2
;
Yao LU
2
;
Shuling WU
2
;
Hongxiao HAO
2
;
Min CHANG
2
;
Leiping HU
2
;
Ruyu LIU
2
;
Yunzhong WU
2
;
Min YANG
2
;
Wenhao HUA
3
;
Shunai LIU
4
;
Minghui LI
2
;
Yao XIE
1
Author Information
- Publication Type:Journal Article
- Keywords: Plasmacytoid dendritic cells; CD4+ T helper cells; Hepatitis B chronic; Acute hepatitis B; Hepatitis B virus
- From: Chinese Journal of Experimental and Clinical Virology 2017;31(6):487-491
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the differences in function of plasmacytoid dendritic cells (pDC) and CD4+ T helper cells (CD4+ Th cells) between acute hepatitis B (AHB) and chronic hepatitis B (CHB).
Methods:In this study, patients with AHB and those with CHB in immune active (IA) phase were enrolled. The frequencies of pDC, CD86+ pDC, CD4+ T cells and their subsets, surface functional molecules were detected respectively among patients with chronic HBV infection in IA phase, patients with AHB, those recovered from AHB. Meanwhile, their correlations with ALT, HBV DNA and HBV markers were analyzed.
Results:The ALT level in AHB was significantly higher than that in IA, and inflammation was more obvious in AHB. Between IA and AHB, CD86+ pDC frequency and the mean fluorescence intensity of functional molecule CD86 (CD86MFI) were higher in IA than those in AHB, but the frequency of CD4+ T cells in AHB was higher than that in IA. For patients who got over AHB, the frequency of CD86+ pDC increased; Th1 were on the rise, while the frequencies of CD4+ T and Th2 decreased after the recovery of AHB, and Th2 / Th1 ratio decreased..In AHB, HBVDNA loads were positively correlated with ALT levels and Th2 frequencies.
Conclusions:In CHB immune active phase, CD86+ pDC with stimulating function played an important role, but the cellular immune response of CD4+ T cells decreased. In AHB inflammatory stage, CD4+ T cells played a strong cellular immune response, which result ed in viral clearance. Th2 cells regulation of CD4+ T cells played a dominant role, which was involved in the inflammatory response, and the cytotoxic role of Th1 cells during the recovery period was dominant, playing a strong cellular immune response, then the virus were completely eliminated.
