Influence of extremely low-frequency magnetic field on circadian rhythm of cryptochrome in mouse embryonic fibroblasts
10.3760/cma.j.issn.1001-9391.2017.06.015
- VernacularTitle: 极低频磁场对小鼠胚胎成纤维细胞中隐花色素生物节律的影响
- Author:
Zhaoyu SUN
1
;
Duyan GENG
;
Chuanfang CHEN
;
Pingping WANG
;
Tao SONG
Author Information
1. Province-Ministry Joint Key Laboratory of Electromagnetic Field and Electrical Apparatus Reliability, Hebei University of Technology, Tianjin 300130, China
- Publication Type:Case Reports
- Keywords:
Cryptochome;
Biorhythm;
Extremely low frequency magnetic fields
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(6):459-462
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of extremely low-frequency magnetic field on periodical expression of cryptochrome (Cry) gene in mouse embryonic fibroblast NIH3T3 cells.
Methods:The NIH3T3 cells were divided into magnetic field group and sham-exposure group. The NIH3T3 cells in the magnetic field group were stimulated by horse serum and then exposed to an extremely low-frequency magnetic field (50 Hz and 0.3 mT) for 48 hours, and those in the sham-exposure group were also stimulated by horse serum and then exposed to a coil for 48 hours. The NIH3T3 cells were collected, total RNA was extracted, and cDNA was obtained via reverse transcription. Real-time fluorescent quantitative RT-PCR was used to measure the changes in transcription cycles of Cry and Period genes in both groups.
Results:There was no significant difference in the proliferation rate at 0, 12, 24, and 48 hours of exposure between the two groups (P>0.05) . Both sham-exposure group and magnetic field group showed a rhythmic change in the expression of Cry gene, and compared with the sham-exposure group, the magnetic field group had a significantly shortened circadian rhythm of Cry gene in NIH3T3 cells (t=2.57, P<0.05) . Both groups had rhythmic and periodical expression of Period gene and there was no significant difference between the two groups (t=0.70, P>0.05) .
Conclusion:Extremely low-frequency magnetic field can significantly shorten the circadian rhythm of Cry gene in mouse embryonic fibroblasts, while there is no significant change in the circadian rhythm of Period gene.
