Study on autophagy in nucleated red blood cells in patients with myelodysplastic syndromes
10.3760/cma.j.issn.0253-2727.2017.05.015
- VernacularTitle: 骨髓增生异常综合征患者有核红细胞自噬水平的研究
- Author:
Liyan YANG
1
;
Huaquan WANG
;
Rong FU
;
Wen QU
;
Erbao RUAN
;
Xiaoming WANG
;
Guojin WANG
;
Yuhong WU
;
Hong LIU
;
Jia SONG
;
Jing GUAN
;
Limin XING
;
Lijuan LI
;
Huijuan JIANG
;
Hui LIU
;
Yihao WANG
;
Chunyan LIU
;
Wei ZHANG
;
Zonghong SHAO
Author Information
1. Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China
- Publication Type:Journal Article
- Keywords:
Myelodysplastic syndromes;
Autophagy;
Anemia
- From:
Chinese Journal of Hematology
2017;38(5):432-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the change of autophagy level of bone marrow nucleated red blood cell (RBC) in patients with myelodysplastic syndromes (MDS) .
Methods:Fifty-four MDS patients and thirty-three controls were enrolled in this study. The mitophagy were observed by transmission electron microscopy (TEM) . The level of autophagy-associated protein LC3B in GlycoA+ nucleated RBC was measured by flow cytometry. The expressions of ULK1 and mTOR mRNA in GlycoA+ nucleated RBC were measured by real-time PCR. The expression of the mitochondrial outer membrane protein TOM20 in GlycoA+ nucleated RBC was detected by Western blot.
Results:Autophagosomes or autolysosomes were scarcely observed by TEM in MDS patients. The expression of LC3B in GlycoA+ nucleated RBC in high-risk MDS patients (0.22±0.12) was significantly lower than that in normal controls (0.43±0.22, P<0.001) , and lower than that in low-risk MDS patients (0.40±0.16, P=0.001) . The expression of AMPK [0.26 (0.60) ] in GlycoA+ nucleated RBC in high-risk MDS patients was significantly lower than that in controls [1.00 (2.07) , P<0.017) . The expression of ULK1 mRNA in GlycoA+ nucleated RBC in high-risk MDS patients [0.27 (3.31) ] was significantly lower than that in controls [1.07 (4.41) , P<0.017]. The level of mTOR mRNA in GlycoA+ nucleated RBC in high-risk MDS patients [1.82 (3.74) ] was significantly higher than that in controls [1.26 (1.38) , P<0.017]. The level of LC3B in GlycoA+ nucleated RBC was negatively correlated with the HGB (r=0.529, P=0.009) in high-risk MDS patients. The expression of mitochondrial outer membrane protein TOM20 in high-risk MDS patients was 9.42±4.42.
Conclusion:Autophagy is impaired in nucleated RBC of MDS patients.
