A Study on the establishment of immune thrombocytopenia model induced by anti-platelet GPⅠbα antibodies
10.3760/cma.j.issn.0253-2727.2017.05.007
- VernacularTitle: 抗血小板GPⅠbα抗体诱导建立原发免疫性血小板减少症模型的研究
- Author:
Kangxi ZHOU
1
,
2
,
3
;
Rong YAN
;
Mengxing CHEN
;
Jun LIU
;
Qingya CUI
;
Renping HU
;
Yancai LIU
;
Yang ZHANG
;
Changgeng RUAN
;
Kesheng DAI
Author Information
1. Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University
2. Key Laboratory of Thrombosis and Hemostasis, Ministry of Health
3. Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, China
- Publication Type:Journal Article
- Keywords:
Thrombocytopenia;
Models, animal;
Platelet Membrane GlycoproteinⅠb
- From:
Chinese Journal of Hematology
2017;38(5):390-393
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish primary immune thrombocytopenia (ITP) animal model induced by anti-platelet membrane glycoprotein GPⅠbα antibodies AN51 and R300.
Methods:Twenty guinea pigs (6-8 week) were divided into 4 groups. Five guinea pigs in each group were intravenously injected with different doses of AN51 (0.05, 0.1, 0.2 μg/g) and 0.2 μg/g IgG as control. The whole blood was collected from inner angular venous plexus. Platelets number was determined by an automated cell counter and Swiss-Jim method. Then, the similar protocol was used to establish ITP nude mice model by intraperitoneal injection of different concentrations of anti-platelet GPⅠbα antibody R300, respectively.
Results:①Five minutes after intravenous injection of AN51 at 0.05, 0.1 and 0.2 μg/g, the platelet counts of guinea pigs reduced about 0-5%, 50%-60% and 70%-80% compared to the control group, respectively. The difference was statistically significant (P<0.01) . ②Six hours after intraperitoneal injection of R300 at 0.05, 0.1, 0.2 μg/g, the platelet counts of nude mice decreased about 20%-30%, 60%-70% and 80%-90% compared to the control group, respectively. The difference was statistically significant (P<0.01) . The nude mice, injected 0.2 μg/g R300 once a day for 2 weeks, showed typical ITP clinical manifestations including large number of petechiaes or ecchymoses on limbs, head and abdomen.
Conclusion:AN51 at 0.2 μg/g and R300 at 0.2 μg/g could establish stable ITP model in guinea pigs and nude mice respectively.
