Copy number aberrations of genes related to extrohepatic metastasis-free survival after operation for hepatocellular carcinoma
10.3760/cma.j.issn.1007-3418.2017.05.008
- VernacularTitle: 肝癌术后无转移生存相关的基因拷贝数变异分析
- Author:
Lingling BAO
1
;
Zhongzheng ZHU
1
;
Bingji WEN
1
;
Xiaoxi WAN
1
;
Yingquan YE
1
;
Lü CHEN
2
;
Songqin HE
1
;
Wenming CONG
3
Author Information
1. Department of Oncology, No.113 Hospital of People’s Liberation Army, Anhui Medical University, Ningbo 315040, China
2. Ningbo University School of Medicine, Ningbo 315211, China
3. Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Neoplasm metastasis;
Gene;
Copy number aberration
- From:Chinese Journal of Hepatology
2017;25(5):349-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the molecular markers of copy number aberrations (CNAs) of genes related to extrohepatic metastasis-free survival after the operation for hepatocellular carcinoma (HCC).
Methods:The CNA status of 20 candidate genes in 66 HCC samples was detected by microarray comparative genomic hybridization. The associations between gene CNAs and extrohepatic metastasis-free survival were evaluated using the Cox regression model, Log-rank test, and Kaplan-Meier survival analysis.
Results:Multivariate Cox analysis revealed that the independent risk factors for metastasis-free survival were MDM4 gain (hazard ratio [HR] = 2.74, 95% confidence interval [CI] = 1.18-6.37, P < 0.05), APC loss (HR = 8.43, 95% CI = 2.48-28.66, P < 0.01), and BCL2L1 gain (HR = 3.45, 95% CI = 1.13-10.52, P < 0.05) and the independent protective factor was FBXW7 loss (HR = 0.32, 95% CI = 0.12-0.89, P < 0.05). By stepwise Cox regression analysis, three CNAs related to metastasis-free survival were screened out: MDM4 gain (HR = 2.71, 95% CI = 1.11-6.64, P < 0.05), APC loss (HR = 7.19, 95% CI = 1.88-27.60, P < 0.005), and FBXW7 loss (HR = 0.16, 95% CI = 0.05-0.46, P < 0.01). There were significant differences in metastasis-free survival rate between the HCC patients with FBXW7 loss and without MDM4 gain or APC loss, those with MDM4 gain and/or APC loss and without FBXW7 loss, and those with other CNA combinations (log-rank test, P < 0.01).
Conclusion:MDM4 gain, APC loss, and FBXW7 loss are the independent prognostic factors for extrohepatic metastasis-free survival after the operation for HCC and can be used to predict the risk of extrohepatic metastasis after the operation for HCC.
