Sorting role of p16INK4a/Ki-67 double immunostaining in the cervical cytology specimens of ASCUS and LSIL cases
10.3760/cma.j.issn.0529-5807.2017.05.008
- VernacularTitle: p16INK4a/Ki-67双免疫细胞化学染色对宫颈细胞学不能明确意义的不典型鳞状细胞和低级别鳞状上皮内瘤变患者的分流作用
- Author:
Jing YU
1
;
Huiting ZHU
;
Jingjing ZHAO
;
Jianzhong SU
;
Yindi XIA
Author Information
1. Department of Pathology, First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 201204, China
- Publication Type:Journal Article
- Keywords:
Genes, p16;
Ki-67 antigen;
Immunohistochemistry;
Cell biology
- From:
Chinese Journal of Pathology
2017;46(5):323-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the sorting effect of p16INK4a/Ki-67 double immunostaining method in patients with atypical squamous cells of undetermined significance (ASCUS) or low-grade squamous intraepithelial lesion (LSIL) cytology results.
Methods:Four-hundred and twenty cases collected during April 2014 to February 2015 of cervical cytology of ASCUS (n=318) and LSIL (n=102) were selected, and residual liquid-based cytology specimens were used for p16INK4a/Ki-67 double immunostaining. The sensitivity and specificity of the detection of cervical precancerous lesions and cervical cancer were calculated, and the results were compared with high risk HPV. Taking histological follow-up as the gold standard, the test was considered positive when at least one cell exhibited p16INK4a/Ki-67 co-staining, without requirement of adjunct morphologic interpretation of positive cells.
Results:Further screening CIN2+ in cytology ASCUS and LSIL group , the sensitivity of p16INK4a/Ki-67 double immunostaining was slightly lower than high risk HPV (84.2% vs. 94.7%), while the specificity was higher (84.0% vs. 53.9%). For ASCUS patients, the sensitivity of p16INK4a/Ki-67 double immunostaining and high risk HPV was 82.6% and 91.3%, and the specificity was 88.8% and 63.7%, respectively. For LSIL patients, the sensitivity of p16INK4a/Ki-67 double immunostaining and high risk HPV was 86.7% and 100.0%, and the specificity was 67.8% and 20.7%, respectively. For patients younger and older than 30 years, specificity of p16INK4a/Ki-67 double immunostaining was both higher than that of high risk HPV (80.8% vs. 42.3%; 84.6% vs. 56.9%).
Conclusions:p16INK4a/Ki-67 double immunostaining can effectively identify the high risk population in ASCUS or LSIL, with higher specificity than high risk HPV test. p16INK4a/Ki-67 double immunostaining may benefit patients younger than 30 years of age as a preliminary or potential cytology-combining screening tool.
