Clinicopathologic characteristics of epidermal growth factor receptor mutations in non-small cell lung cancer in Xinjiang region
10.3760/cma.j.issn.0529-5807.2017.05.005
- VernacularTitle: 新疆地区非小细胞肺癌中表皮生长因子受体基因突变与临床病理特征的关系
- Author:
Yi SHI
1
;
Zheng PAN
2
;
Wenli CUI
;
Zhiping MA
;
Xuelian PANG
;
Wei ZHANG
;
Yuqing MA
Author Information
1. Department of Pathology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830011, China
2. Department of Pathology, Xinjiang Uygur Autononmous Rejion Occupational Disease Hospital, Urumqi 830091, China
- Publication Type:Journal Article
- Keywords:
Carcinoma, non-small-cell lung;
Receptor, epidermal growth factor;
DNA mutational analysis;
Polymerase chain reaction;
DNA probes
- From:
Chinese Journal of Pathology
2017;46(5):309-313
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the point mutation of epidermal growth factor receptor (EGFR) gene and clinicopathologic characteristics in patients with non-small cell lung cancers(NSCLC)of Xinjiang region.
Methods:Five-hundred and eighty-two cases of paraffin-embedded tissue in patients with NSCLC were collected between January 2013 and December 2015 in the First Affiliated Hospital of Xinjiang Medical University. The DNA was extracted from these tissues by Qiagen kit, to test thirty-two mutations in EGFR exons 18, 19, 20 and 21 using fluorescent quantitative qRT-PCR technology by TaqMan probe; the clinicopathologic features of patients were analyzed according to the mutation status of EGFR.
Results:There were 173 cases with EGFR gene mutation in 582 cases of paraffin-embedded tissue in patients with NSCLC, and the mutation rate was 29.7%(173/582). There were statistical difference in female patients (50.5%, 98/194), no history of smoking(47.3%, 96/203), high differentiation(6/9), adenosquamous carcinoma(6/11), peripheral location (34.9%, 88/252), and surgical specimens(38.2%, 83/217), respectively (P<0.05). Multiple factors Logistic analysis showed that gender, degree of differentiation, and pathologic types had statistical differences to EGFR when α=0.05. There were no statistical differences between other variants.
Conclusions:There are higher rate EGFR gene mutation in women patients, non-smokers, and well-differentiated, adenocarcinoma. Gender, degree of differentiation and pathological patterns are independent influencing factors on EGFR mutation status.