Detection of six main species of viruses in human cell lines
10.3760/cma.j.issn.1003-9279.2017.05.018
- VernacularTitle: 人源细胞系中6种主要病毒的检测
- Author:
Zhenli YANG
1
;
Xiaocui BIAN
;
Hailiang FENG
;
Yuqin LIU
Author Information
1. Cell Resource Center, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China
- Publication Type:Journal Article
- Keywords:
Cell lines;
Virus;
Polymerase chain reaction;
Electron microscopy
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(5):457-461
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clarify the virus hosting status of human cell lines.
Methods:The DNA of Epstein-Barr virus (EBV), Hepatitis B virus (HBV), Human pappilomavirus (HPV), Human immunodeficiency virus (HIV), Human T-cell leukemia virus (HTLV) in 135 human cell lines were checked using PCR, and HCV RNA sequences were checked by RT-PCR. The transmission electron microscopy (TEM) was used to examine the virus particles in cells. The high-risk genotypes of HPV were tested by PCR.
Results:By PCR assaying, among the 135 human cell lines, four cell lines(Daudi, Raji, EB-3, NCI-BL2009) harbored EBV DNA sequences; two(Hep3B, PLC/PRF/5) harbored HBV DNA sequences; seven (HeLa, HeLa 229, H1HeLa, HeLaS3, SiHa, Caski, CNE-2Z) harbored HPV DNA sequences, including two cell lines (SiHa, Caski) with HPV16, five cell lines(HeLa, HeLa 229, H1HeLa, HeLaS3, CNE-2Z) with HPV18; one cell line(HUT 102) harbored HTLV DNA sequences. No cell line harbored HCV RNA sequences and HIV DNA sequences. No viral particles were observed in the positive cell lines by TEM, but some viral inclusion bodies in certain cell lines.
Conclusions:The virus hosting status of human cell lines can be checked by PCR or RT-PCR. The viral DNA sequences were integrated in the cellular genome.
