Establishment of a real-time PCR method to identify Ekpoma virus gene in blood sample of a returnee from Angola

VernacularTitle: 建立荧光定量PCR方法鉴定1例入境人员携带伊科普玛病毒基因
Author: Dong XIA ¹ ; Juan SONG ; Xiaonuan LUO ; Qinqin SONG ; Xinling WANG ; Guizhen WU ; Jun HAN
Author Information

1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Publication Type:Journal Article
Keywords: Ekpoma virus; Quantitative real-time polymerase chain reaction
From: Chinese Journal of Experimental and Clinical Virology 2017;31(5):454-456
CountryChina
Language:Chinese
Abstract: Objective:To establish quantitative real-time PCR (qPCR) method based on Taqman probe for detecting Ekpoma virus (EKV).
Methods:According to the conserved region of gene in EKV genome from GenBank, primers and probe for qPCR were designed. Validity and sensitivity were evaluated in this study. Both whole blood and serum of a returnee from Angola were tested by the established EKV-1 and EKV-2 qPCR method .
Results:Sensitivity of EKV-1 and EKV-2 qPCR method was respectively 41 copies/μl and 70 copies/μl. Coefficient of variance (CV) was respectively 1.27%, 0.20%, 0.82%; 2.12%, 1.74%, and 1.40%. EKV-2 gene was detected in both whole blood and serum of a returnee from Angola.
Conclusions:The first EKV-2 gene was confirmed in both whole blood and serum of a returnee from Angola by real-time RT-PCR..