Molecular diagnosis of yellow fever imported into Fujian province, 2016

Yuwei Weng; Jinzhang Wang; Yongjun Zhang; Jianming OU; Rongtao Hong; Shengcan Guan; Zhong Lin; Lifeng Han; Kuicheng Zheng; Linglan Wang; Yansheng Yan

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Molecular diagnosis of yellow fever imported into Fujian province, 2016

VernacularTitle: 2016年福建省输入性黄热病的分子诊断
Author: Yuwei WENG ¹ ; Jinzhang WANG ¹ ; Yongjun ZHANG ¹ ; Jianming OU ¹ ; Rongtao HONG ¹ ; Shengcan GUAN ² ; Zhong LIN ¹ ; Lifeng HAN ² ; Kuicheng ZHENG ¹ ; Linglan WANG ¹ ; Yansheng YAN ¹
Author Information

1. Fujian Center for Disease Control and Prevention, Fujian Provincial Key Laboratory of Zoonosis, Fuzhou 350001, China
2. Fuzhou Mengchao Hepatobiliary Hospital of Fujian Medical University. Fuzhou 350025, China
Publication Type:Journal Article
Keywords: Yellow fever; Imported case; Molecular diagnosis; Vaccine
From: Chinese Journal of Experimental and Clinical Virology 2017;31(5):441-445
CountryChina
Language:Chinese
Abstract: Objective:To make laboratorial diagnosis of imported yellow fever (YF) cases in Fujian province with molecular method .
Methods:Serum and urine samples were collected from suspected cases at various time-points post illness onset. Real-time RT-PCR and nested RT-PCR were performed respectively for viral specific nucleotide detection and fragment amplification. Sequencing and restrictive fragment length polymorphism (RFLP) method were used to identify the wild virus infection.
Results:A total of five cases with wild yellow fever virus (YFV) infection were confirmed in this study. It revealed that the viral agent belonged to Angola-71 like YFV, and the duration of viral agent in urine was longer than that in serum.
Conclusions:Simultaneous detection of serum and urine samples would increase detection sensitivity, and further RFLP method contributed to rapid identification of wild YFV infection and exclusion of positive result due to recent vaccination.