Detection of four human coronaviruses in respiratory infections in children in Fuzhou, China
10.3760/cma.j.issn.1003-9279.2017.05.011
- VernacularTitle: 福州地区重症呼吸道感染患儿中4种人冠状病毒的检测与分析
- Author:
Wenqiong XIU
1
;
Kuicheng ZHENG
1
;
Bingshan WU
1
;
Meng HUANG
1
;
Jianfeng XIE
1
;
Yulan KANG
2
;
Guanghua LIU
2
Author Information
1. Fujian Center for Disease Control and Prevention Fujian Key Laborotary of Zoonoses, Fuzhou 350001, China
2. Fujian Provincial Maternity and Child Health Hospital, Fujian Medical University, Fuzhou 350001, China
- Publication Type:Journal Article
- Keywords:
Respiratory tract infections;
Human coronavirus;
HCoV-HKU1;
HCoV-NL63;
HCoV-OC43;
HCoV-229E
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(5):429-433
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In this study, we tested for the presence of four human coronaviruses (HCoVs) in children with respiratory tract disease in Fuzhou, Fujian, China.
Methods:Nasopharyngeal aspirates were collected from children with respiratory tract disease from Nov, 2007 to Jan, 2015. A total of 266 clinical samples were tested for HCoVs using reverse-transcription polymerase chain reaction (RT-PCR). The positive products were sequenced and compared with those in GenBank by BLAST. The positive samples were then tested for HCoV-HKU1 and HCoV-NL63 using RT-PCR method . We compared the 440 bp pol gene sequence of the 8 HCoV isolates in Fuzhou, China to other HCoV isolates documented in the GenBank database by using MEGA software version 6.06 and the neighbor-joining method .
Results:HCoVs were detected in 8 patients (3.0%) out of the 266 children. Two of 266 (0.38%) were positive for HCoV-HKU1; 1 of 266(0.38%)were positive for HCoV-NL63; 1 of 266 (0.38%) were positive for HCoV-229E; 4 of 266 (1.5%)were positive for HCoV-OC43. All of children who were positive for HCoV had respiratory illness. Two HCoV-HKU1 were found to co-infect with human parainfluenza virus type 3 (HPIV-3). The 8 HCoV strains in our study fell into four clusters. Two strains of HCoV-HKU1 were genotype A.
Conclusions:HCoV infections were probably associated with upper and lower respiratory illness in children. Additional studies are needed to investigate the potential roles of these HCoVs in diseases.
