Analysis of hepatitis C virus 5′ non-coding region for viral genotyping and the level of viral replication
10.3760/cma.j.issn.1003-9279.2017.05.008
- VernacularTitle: HCV 5′非编码区基因分型与病毒复制水平的分析
- Author:
Yue WANG
1
;
Jin′e LEI
2
;
Wei DUAN
3
;
Xiao JIANG
4
;
Lijun MU
4
;
Lingyun HUI
2
;
Wenxin SHI
4
;
Congya ZHOU
4
;
Yihua DU
1
Author Information
1. Department of Pathogenic Microbiology and Immunology, School of Basic Medical Sciences, Xi′an Jiaotong University, Xi′an 710061, China
2. Department of Clinical Laboratory, the First Affiliated Hospital of Xi′an Jiaotong University, Xi′an 710061, China
3. Xi′an Central Hospital, Xi′an 710000, China
4. School of Basic Medical Sciences, Xi′an Jiaotong University, Xi′an 710061, China
- Publication Type:Journal Article
- Keywords:
Hepatitis C Virus;
5′non-cording region;
HCV genotyping;
Serum HCV-RNA concentration
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(5):414-418
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate hepatitis C virus(HCV)genotyping and the serum HCV-RNA concentration in patients infected with different HCV genotypes and to provide information for evaluation of disease condition and anti-viral treatment efficacy.
Methods:A total of 60 anti-HCV positive serum samples were collected before antiviral treatment. RT-PCR was performed for the 5′ non-cording region and was followed by nucleotide sequencing for HCV genotyping. Meanwhile, serum HCV-RNA concentration was detected by quantitative PCR. SPSS21.0 and Graphpad Prism 5.0 software were used for data analysis. Analysis of variance (ANOVA) was used for comparison among multi-groups and the t-test was used for comparison between two groups.
Results:The frequencies of HCV genotypes 1b, 3a, 1a and 2a were 48.3% (29/60), 23.3% (14/60), 16.7% (10/60) and 10% (6/60), respectively. And, there is one subtype 2c was detected in this study. The mean serum viral concentration with standard deviation of HCV in genotype 1a, 1b, 2a, and 3 a were 5.46±1.19, 6.22±0.78, 5.47±0.65, and 5.38±0.98 log10 (IU/ml) respectively.
Conclusions:The infection rate of HCV genotype 1 was significantly higher than that of genotype 2 and 3 (P<0.01). The statistical analysis showed the serum HCV-RNA concentration in patients with subtype 1b was significantly higher than that of the subtype group 1 a, 2 a, and 3 a (P<0.05). The study of the relationship between HCV genotypes and the serum HCV-RNA concentration may contribute to anti-viral treatment prescription for hepatitis C patients.
