Isolation and identification of Getah virus from Culicoides in Yunnan
10.3760/cma.j.issn.1003-9279.2017.05.006
- VernacularTitle: 库蠓中盖塔病毒的分离及其分子鉴定
- Author:
Pei DONG
1
;
Nan LI
2
;
Yuwen HE
2
;
Tiangang XU
3
;
Jianzhong YIN
1
;
Jinglin WANG
2
Author Information
1. School of Public Health, Kunming Medical University, Kunming 650500, China
2. Yunnan Key Laboratory of Tropical and Subtropical Sciences of Yunnan Animal Science and Veterinary Institute, Kunming 650224, China
3. China Animal Health and Epidemiology Center, Qingdao 266032, China
- Publication Type:Journal Article
- Keywords:
Getah virus;
Culicoides;
Isolation;
Identification
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(5):405-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the taxonomy and molecular characteristics of one virus strain (SZC30) isolated from Culicoides in Yunnan.
Methods:Culicoides were collected with light trap method in the Wulong Village of Shizong County of Yunnan Province in July, 2013. BHK-21 and C6/36 cells were used for virus isolation. The positive isolates were inoculated into brain of one-day suckling mice. Alphavirus and Getah virus specific primers were used to amplify the genome of the virus isolation by RT-PCR. The products of RT-PCR were sequenced. Clustal X1.83, DNAStar, Mega5.1 were used for bioinformatics analysis.
Results:Totally 3 500 culicoides were collected and divided into 41 batches for virus isolation. One isolate (SZC30) produced cytopathic effect (CPE) on BHK-21 and C6/36 cells; the result of RT-PCR with Alphavirus and Getah virus NS1 specific primer were positive; the sequence analysis of NS1 gene suggested that SZC30 and two Getah virus strains (YN0540, SC1210) from China were in the same evolutionary branching, the nucleotide homology were 97%-100%, and the amino acid was 97%-100%.
Conclusions:SZC30 isolated in Yunnan province was identified as Getah virus.
