Effect of mandibular advancement device upon nuclear factor κB and tumor necrosis factor α, interleukin 6 in genioglossus of rabbit with obstructive sleep apnea hypopnea syndrome
10.3760/cma.j.issn.1002-0098.2017.05.008
- VernacularTitle: 下颌前移矫治器对阻塞性睡眠呼吸暂停低通气综合征兔颏舌肌核因子κB、肿瘤坏死因子α和白细胞介素6的影响
- Author:
Shilong ZHANG
1
;
Chunyan LIU
1
;
Wen WANG
1
;
Xing QIAO
1
;
Haiyan LU
1
Author Information
1. Department of Orthodontics, College of Stomatology, Hebei Medical University, Shijiazhuang 050017, China
- Publication Type:Journal Article
- Keywords:
NF-kappa B;
Tumor necrosis factor-alpha;
Obstructive sleep apnoea hypopnea syndrome;
Mandibular advancement device
- From:
Chinese Journal of Stomatology
2017;52(5):300-304
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of mandibular advancement device (MAD) upon nuclear factor κB (NF-κB), tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) in the genioglossus.
Methods:Eighteen New Zealand white rabbits (male, six months old), in accordance with the random number table, were equally divided into three groups, the control group, obstructive sleep apnea hypopnea syndrome (OSAHS) group and MAD group. All animals were induced to sleep in supine position for 2 hours every morning in the next 8 weeks. The specimens of genioglossus were prepared. The relative expression of NF-κB p65 was measured with Western blotting and the mass concentration of TNF-α and IL-6 was determined with enzyme-linked immunosorbent assay.
Results:The relative expressions of NF-κB p65 protein in genioglossus in the control group, OSAHS group and MAD group were 0.24±0.07, 0.44±0.08 and 0.30±0.09, respectively. The mass concentrations of TNF-α in genioglossus in the control group, OSAHS group and MAD group were (0.065±0.020), (0.097±0.018) and (0.071±0.020) μg/L, respectively. The mass concentrations of IL-6 in genioglossus in the control group, OSAHS group and MAD group were (0.063±0.013), (0.093±0.017), and (0.069±0.014) μg/L, respectively. For the above indicators, the data in OSAHS group were all significantly higher than that in MAD group and the control group (P<0.05). No significant difference was found between MAD group and the control group (P>0.05).
Conclusions:Treatment of OSAHS with MAD decreased the mass concentration of TNF-α and IL-6 leading to fatigue of genioglossus, reduced the activation of NF-κB and played a significant role in protecting genioglossus.
