The effect of aluminum trichloride on expression of phosphorylated tau and Aβ in SH-SY5Y cells
10.3760/cma.j.issn.1001-9391.2017.05.011
- VernacularTitle: 铝和ApoEε4等位基因对神经母细胞瘤细胞磷酸化tau蛋白和Aβ表达的影响
- Author:
Xiaoting LU
1
;
Hao WANG
;
Zhijian JIA
;
Qiuying LI
;
Qiao NIU
Author Information
1. Department of Occuoational Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, China
- Publication Type:Case Reports
- Keywords:
Aluminum;
Alleles;
Neuroblastoma;
Gene expression
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(5):359-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of phosphorylated tau (p-tau) and Aβ in SH-SY5Y cells induced by aluminum or/and ApoE ε4 allele, and study the interaction between aluminum and ApoE ε4 allele.
Methods:SH-SY5Y cells were assigned to control group, 400 μmol/L AlCl3 group, ApoEε4 transfected group and 400 μmol/L AlCl3 with Apo Eε4 transfected group. The cell viability was measured by CCK-8 assay; the expressions of p-tau and Aβ was determined with ELISA Kit after AlCl3 exposure or or/and ApoE ε4 transfection.
Results:The viability of cells exposed to 400 μmol/L AlCl3 or/and ApoE ε4 transfected were significantly lower than that of controls (P<0.05) . The expressions of total tau, tau-181, tau-231, tau-396 and Aβ in 400 μmol/L Al3+ or/and ApoE ε4 transfected exposed cells showed significantly higher than those of controls (P<0.05) . Based on the factorial design, a significant interaction exists, and there is a synergistic effect between AlCl3 and ApoE ε4 (P<0.05) .
Conclusion:Aluminum and ApoE ε4 allele could increase expression of p-tau and Aβ deposition; there was a synergistic interaction between aluminum and ApoE ε4 allele on cell death, tau phosphorylation and Aβ deposition of SH-SY5Y.
