Caspase 3 and Ki-67 Immunoreactivity and Its Correlation with Frequency of Apoptosis in Gastric Adenomas and Carcinomas.
- Author:
Jin Hee SOHN
1
;
Seoung Wan CHAE
;
Kyung Chan CHOI
;
Hyung Sik SHIN
Author Information
1. Department of Pathology, Hallym University, College of Medicine, Seoul 150-020, Korea. jhsohn@www.hallym.or.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Caspases;
Ki-67 antigen;
Carcinoma;
Stomach
- MeSH:
Adenoma*;
Apoptosis*;
Caspase 3*;
Caspases;
Cell Death;
Genes, Tumor Suppressor;
Immunohistochemistry;
Ki-67 Antigen;
Oncogenes;
Peptide Hydrolases;
Stomach
- From:Korean Journal of Pathology
2001;35(4):286-290
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Apoptosis, also known as programmed cell death, is under genetic control and is mediated by apoptosis-specific genes, certain oncogenes and tumor suppressor genes. Caspase 3, a group of cystein proteases, is involved in the induction of apoptosis and has been considered to be correlated with apoptosis. Therefore, we tried to define whether caspase 3 is expressed in gastric adenoma and carcinoma, and correlated with apoptosis. METHODS: The apoptotic index and caspase 3 and Ki-67 immunoreactivity were observed in 25 gastric adenomas, 31 early gastric carcinomas (EGC) and 64 advanced gastric carcinomas (AGC) by in situ labelling and immunohistochemistry. RESULTS: The mean number of apoptotic bodies and caspase 3 immunoreactivity were significantly increased from adenoma through EGC to AGC. Ki-67 immunoreactivity was more increased in AGC than in adenoma and EGC. And the number of apoptotic bodies were positively correlated with caspase 3 and Ki-67 immunoreactivity, and caspase 3 immunoreactivity was negatively correlated with Ki-67 immunoreactivity even though they were statistically insignificant. CONCLUSIONS: Our results suggest that caspase 3 activation is important for inducing apoptosis, and both caspase 3 and apoptosis are increased along the tumor progression.
