Regulatory effect of faciogenital dysplasia 6 gene on hepatic stem cell differentiation
10.3760/cma.j.issn.1007-3418.2017.04.007
- VernacularTitle: Faciogenital dysplasia 6基因对肝干细胞分化的调控作用
- Author:
Qian XUE
1
;
Jinfeng YANG
1
;
Bing LI
1
;
Tongchuan HE
2
;
Bingqiang ZHANG
1
Author Information
1. Department of Gastroenterology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
2. Molecular Oncology Laboratory, the University of Chicago Medical Center, Chicago 60637, USA
- Publication Type:Journal Article
- Keywords:
Stem cells;
RNA interference;
HP14.5 cells;
Hepatic progenitor cells differentiation;
Faciogenital dysplasia
- From:
Chinese Journal of Hepatology
2017;25(4):268-272
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulatory effect of faciogenital dysplasia 6 (FGD6) gene on hepatic stem cell differentiation.
Methods:FGD6 gene was selected for the co-intervention of target sequence, the AdEasy system was used for the construction of adenovirus vector and the packaging and multiplication of the recombinant adenovirus vector pSES-FGD6-siRNA, and the HP14.5 cells were infected. Immunofluorescence assay was used to measure the expression of FGD6 protein in HP14.5 cells, quantitative real-time PCR was used to measure the mRNA expression of FGD6, alpha-fetoprotein (AFP), and albumin (Alb), and Western blot was used to measure the protein expression of FGD6, AFP, and Alb. The empty pSES-Ad-RFP adenovirus vector was constructed as control in each group. All data were expressed as x±s, and a one-way analysis of variance was performed.
Results:FGD6 protein was mainly expressed in the nucleus of HP14.5 cells. The pSES-FGD6-siRNA adenovirus vector was successfully constructed and it downregulated the expression of FGD6 gene and the mRNA and protein expression of AFP in HP14.5 cells and upregulated the mRNA and protein expression of Alb (P < 0.01).
Conclusion:The inhibition of the expression of FGD6 gene in HP14.5 cells may differentiate HP14.5 cells into hepatocytes. Therefore, FGD6 gene plays an important role in the differentiation regulation of hepatic stem cells.
