Establishment and application of micro-neutralization test to determine neutralizing antibody against ZIKA virus in sera
10.3760/cma.j.issn.1003-9279.2017.04.020
- VernacularTitle: 人血清中寨卡病毒微量中和抗体检测方法的建立和应用
- Author:
Xunmin JI
1
;
Juan SU
2
;
Lina YI
3
;
Tuohua PENG
1
;
Qiqi TAN
2
;
Huan ZHANG
2
;
Jiufeng SUN
3
;
De WU
2
;
Xianchang ZHANG
1
;
Changwen KE
2
Author Information
1. Guangdong Provincial Institute of Biological Products and Materia Medica, Guangzhou 510440, China
2. Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China
3. Guangdong Provincial Institute of Public Health, Guangzhou 511430, China
- Publication Type:Journal Article
- Keywords:
Neutralizing antibody;
ZIKA virus;
VERO cell line
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(4):367-371
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a micro-neutralization test for determination of neutralizing antibody against ZIKA virus (ZIKV) in human sera and to verify the acute and convalescent serum samples of 10 ZIKA virus-infected cases diagnosed by nucleic acid detection and/or virus isolation.
Methods:ZIKV isolated from ZIKA cases was used to determine micro-neutralization antibody. The virus solution was prepared by infecting BHK21, VERO and VERO-E6 cell lines and viral titer was tested; 100 TCID50 viral solution and 4 times diluted sera which were inactivated at 56 ℃ for 30 min were neutralized, then added the cell suspension and incubated in 5% CO2 incubator at 37 ℃ for 7 d. The CPE was observed every day.
Results:The sensitivity of BHK21, VERO and VERO-E6 was different after infection with ZIKA virus. VERO cell line was the most sensitive and showed typical CPE. VERO cell line was used to establish a micro-neutralization test for determination of neutralizing antibody against ZIKA virus in sera.
Conclusions:The neutralizing antibody test for zika virus in sera is a special and usefulmethod to diagnose human infection of ZIKV and to conduct population based epidemiological investigation.
