Rapid detection of human adenovirus by recombinase polymerase amplification assay and lateral flow dipstick
10.3760/cma.j.issn.1003-9279.2017.04.018
- VernacularTitle: 重组酶聚合酶扩增结合横向流体试纸条快速检测人腺病毒
- Author:
Kangchen ZHAO
1
;
Yiyue GE
;
Lunbiao CUI
;
Yin CHENG
;
Zhiyang SHI
;
Fengcai ZHU
;
Minghao ZHOU
Author Information
1. Key Laboratories of Enteric Pathogenic Microbiology of National Health and Family Planning Commission, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China
- Publication Type:Journal Article
- Keywords:
Adenovirus;
Rapid detection;
Recombinase polymerase amplification;
Lateral flow dipstick
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(4):357-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a rapid and sensitive isothermal amplification assay for the detection of human Adenovirus.
Methods:Primers and probe used for recombinase polymerase amplification(RPA)were designed based on the conserved region of the adenoviruses hexon gene. After optimizing the reaction temperature and times, the products of RPA were detected by capillary electrophoresis and lateral flow dipstick(LFD). Sensitivity and specicity of the assay were evaluated. The diagnostic value of the RPA-LFD assay was verified using clinical samples which were simultaneously tested by real time PCR assay.
Results:The analytical sensitivity of RPA-LFD assay was 2 copies DNA molecules per reaction and no cross reaction with other pathogens was observed. Compared with real-time PCR assay, the sensitivity, and specificity of the present assay were all 100%.
Conclusions:The RPA-LFD assay developed in this study has the characteristics of high specificity, sensitivity, rapid and no requirement of expensive equipment which provided a new tool for rapid detection of human adenovirus.
