Role of HCMV infection in the activation and oxidative stress of cultured human umbilical vein endothelial cells
10.3760/cma.j.issn.1003-9279.2017.04.005
- VernacularTitle: HCMV感染致培养脐静脉内皮细胞活化和氧化应激的作用
- Author:
Houzhen TUO
1
;
Jingjing CHE
1
;
Lei LIU
2
;
Jiawei WANG
2
;
Zijing FENG
1
;
Jun CHEN
1
;
Jimei LI
1
;
Yongbo ZHANG
1
;
Dexin WANG
1
Author Information
1. Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
2. Department of Neurology, Tongren Hospital, Capital Medical University, Beijing 100730, China
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Cytomegalovirus;
Vascular cellular adhesion molecule-1;
HUVECs;
Oxidative stress
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(4):298-301
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of the activation and oxidative stress of cultured human umbilical vein endothelial cells (HUVEC) after HCMV infection.
Methods:HUVECs were divided into four groups: control, HCMV(+ ), after HCMV AD169 infection, and the supernatant of the culture was extracted, and the cells were lysed. The levels of vascular cellular adhesion molecule-1 (VCAM-1) in HUVEC were measured by real-time PCR. And the content of nitrogen monoxide (NO) of the supernatant was detected by nitrate reductasemethod accordingly.
Results:Twenty-four hours after infection, the mRNA expression of VCAM-1 in HUVECs of HCMV infected group increased obviously compared to control, and NO quantity increased accordingly and time-dependently. There was significant difference between groups(P<0.01).
Conclusions:HCMV increases the mRNA expression of VCAM-1 and quantity of NO, which may contribute to the formation of AS.
