Prediction and identification of immunodominant linear B cell epitopes in N9 of avian influenza virus H7N9
10.3760/cma.j.issn.1003-9279.2017.04.002
- VernacularTitle: 禽流感病毒H7N9神经氨酸酶的优势线性B细胞抗原位点的预测及确定
- Author:
Meng GAO
1
;
Hui XIE
1
;
Limin JIANG
1
;
Limei GAO
1
;
Hongxia NI
2
;
Yongneng LUO
1
Author Information
1. Institute of Viral Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China
2. Ningbo Center for Disease Control and Prevention, Ningbo 310010, China
- Publication Type:Journal Article
- Keywords:
Influenza A virus;
Linear B cell epitope;
Peptide enzyme-linked immunosorbent assay
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(4):287-290
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify immunodominant linear B cell epitopes in neuraminidase of avian influenza virus H7N9.
Methods:By using protean algorithms of bioinformatic software DNAStar, antigenicity, hydrophilicity and surface probability of the H7N9 neuraminidase sequence was analyzed and their corresponding average indexes were calculated. Multiple regions containing potential linear B cell epitopes were predicted. Corresponding peptides were synthesized artificially and used in peptide-ELISA individually to check their reactivity to confirmed H7N9 positive human sera, and H7N9 negative human sera was used as control.
Results:Seven potential linear B cell epitopes, namely A to G, were predicted according to their relatively strong antigenicity, hydrophilicity and surface probability. All corresponding synthetic peptides reacted strongly with H7N9 positive sera.
Conclusions:Immunodominant linear B cell epitopes in neuraminidase of H7N9 were successfully predicted and confirmed. It will facilitate to clarify molecular basis of the antigen specificity and to make respective antibodies.
