Role of complement regulatory protein CD55 in the liver immune injury of trichloroethylene-sensitized mice
10.3760/cma.j.issn.1001-9391.2017.04.002
- VernacularTitle: 补体调节蛋白CD55在三氯乙烯致敏小鼠免疫性肝损伤中的作用
- Author:
Xian WANG
1
;
Cheng ZHANG
;
Xiaodong YANG
;
Bodong LI
;
Dandan ZANG
;
Peng YANG
;
Jiaxiang ZHANG
;
Qixing ZHU
Author Information
1. Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Hefei 230032, China
- Publication Type:Journal Article
- Keywords:
Trichloroethylene;
Mice;
CD55;
Liver
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(4):246-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of CD55 in liver tissue of trichloroethylene-sensitized mice and discuss the role of CD55 in the liver immune injury of trichloroethylene-sensitized mice.
Methods:6-8 weeks specific pathogen free female BALB/c were randomly divided into blank control group, solvent control group and TCE treatment group to establish BALB/c mice sensitized model. According to mouse skin sensitization reaction score, TCE treatment mice were divided into sensitized and non-sensitized group at 24 h after the last challenge. At 48 h after the last challenge, the blood and aseptic livers were collected. The level of serum ALT was tested by automatic biochemical analyzer and pathology of the liver was observed. C5b-9 deposition was studied by immunohistochemistry (IHC) . CD55 protein expression level in liver tissue was studied by immunohistochemistry and Western blotting. The expression of CD55 mRNA in liver tissue was detected by qRT-PCR.
Results:Liver function test result showed level of serum ALT in TCE sensitized group was significantly higher than solvent control group and TCE non-sensitized group (P<0.05) . There was ballooning degeneration and necrosis of liver cells in TCE sensitized group. IHC demonstrated that TCE sensitized group had obviously increased content of C5b-9 but had reduced content of CD55 compared with solvent control group and TCE non-sensitized group (P<0.05) . Western blotting also showed that TCE sensitized group had lower expression of CD55 than solvent control group and TCE non-sensitized group (P<0.05) . qRT-PCR showed that CD55 mRNA expression level in liver tissue of TCE sensitized group was apparently lower than solvent control group and TCE non-sensitized group (P<0.05) .
Conclusion:Complement activation may be involved in TCE-induced liver injury, and the expression change of complement regulatory protein CD55 may play essential role in the process.
