PD-1/PD-L1 expression and its implications in patients with chronic lymphocytic leukemia
10.3760/cma.j.issn.0253-2727.2017.03.005
- VernacularTitle: PD-1/PD-L1共刺激分子在慢性淋巴细胞白血病患者中的表达及临床意义
- Author:
Jinhua LI
1
;
Nannan PANG
;
Zhenghao ZHANG
;
Rui ZHANG
;
Gang CHEN
;
Jianhua QU
Author Information
1. Xinjiang Medical University First Affiliated Hospital Blood Disease Center, Xinjiang Uygur Autonomous Region of Hematology, Urumqi 830054, China
- Publication Type:Journal Article
- Keywords:
Leukemia, lymphocytic, chronic, B-cell;
Programmed cell death 1 receptor;
Antigens, CD274
- From:
Chinese Journal of Hematology
2017;38(3):198-203
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the expression levels of PD-1/PD-L1 costimulatory molecules and explore the clinical significance in patients with chronic lymphocytic leukemia (CLL) .
Methods:The expression of PD-1/PD-L1 in peripheral blood CD8+ T cells, CD4+T cells, CD19+B, and dendrites cells (DC) was detected by flow cytometry in 57 CLL patients and 20 healthy controls. The correlations of PD-1/PD-L1 expression with disease stage, CD38 expression, ZAP-70 expression, chromosome karyotype abnormality and β2-MG expression were analyzed.
Results:①Compared with control, CLL patients, including 39 males and 18 females with the median age of (63.7±10.7) years, had no statistically significant difference in age and gender (P>0.05) . CLL patients had the higher PD-1/PD-L1 expression than healthy controls (P<0.05) . ②In Rai staging, the later the stage, the higher expression of PD-1/PD-L1 (P<0.05) . ③PD-1 expression in CD8+CD38+ group (11 cases) was higher than that in CD8+CD38- group (46 cases) (P=0.004) , and CD8+ poor prognosis chromosome group (14 cases) also had significant higher PD-1 expression than CD8+good prognosis chromosome group (28 cases) (P=0.004) . ④The expression of PD-L1 was higher in CD38+group, ZAP-70+group, and poor prognosis group, as compared to that in CD38-group (P=0.002) , in ZAP-70-group (P<0.001) , in good prognosis group (P=0.023) . There was no correlation between the expression of PD-1/PD-L1 and β2-MG (P>0.05) .
Conclusion:This data reveals that PD-1/PD-L1 was highly expressed in CLL patients. Its expression levels were correlated with Rai stage, CD38, ZAP-70, chromosome karyotype, but not with β2-MG. PD-1/PD-L1 may be a prognostic factor in patients with CLL.
