Expression of microRNA-221 and IL-17 in papillary thyroid carcinoma and correlation with clinicopathologic features
10.3760/cma.j.issn.0529-5807.2017.03.004
- VernacularTitle: microRNA-221及白细胞介素-17在甲状腺乳头状癌中的表达及相关性
- Author:
Xinglian JIANG
1
;
Hui ZHANG
;
Yanlin CHEN
;
Li PENG
Author Information
1. Department of Pathology, School of Basic Medical Science, Chongqing Medical University, Chongqing 400016, China
- Publication Type:Journal Article
- Keywords:
Thyroid neoplasms;
MicroRNAs;
Interleukin-17
- From:
Chinese Journal of Pathology
2017;46(3):160-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of microRNA-221(miR-221) and IL-17 in papillary thyroid carcinoma (PTC) and their roles in the carcinogenesis of PTC.
Methods:Real-time RT-PCR, Western blot and immunohistochemistry (IHC) were used to detect the expression of miR-221 and IL-17 in 40 cases of PTC, 20 adjacent normal thyroid tissues and 20 cases of nodular goiter, and the correlation with clinicopathologic features was analyzed.
Results:(1)The expression level of miR-221 was significantly higher in PTC compared with nodular goiter and adjacent normal thyroid tissue (P<0.05), but not between the latter two (P>0.05). The expression of miR-221 was related to TNM staging, capsular invasion and lymph node metastasis (P<0.05) but not to patients′ age, sex, tumor size, multifocality, tumor spread and vascular invasion.(2)Real-time RT-PCR and Western blot showed that higher levels of IL-17 mRNA and IL-17 protein in PTC than nodular goiter and adjacent normal thyroid tissue (P<0.05), but not between the latter two (P>0.05). IHC assay showed positive expression of IL-17 in PTC but not in nodular goiter and adjacent normal thyroid tissue. IL-17 expression was related to TNM staging, capsular invasion and lymph node metastasis, but not to patients′ age, sex, tumor size, tumor spread and vascular invasion.(3) Expression analysis revealed a significant correlation between the miR-221 and IL-17 expression (r=0.524, P=0.001).
Conclusions:The expressions of miR-221 and IL-17 are significantly higher and positively correlated in PTC, suggesting that miR-221 may regulate IL-17 expression by interacting with a variety of cytokines and inflammatory cells to participate in the development of PTC. miR-221 may be a potential novel prognostic indicator and therapeutic target for PTC.
