Biological characteristics of synovial-derived mesenchymal stem cells in serum-free culture media and in vitro construction of three-dimensional cartilage in vitro
10.3760/cma.j.issn.1009-4598.2017.03.012
- VernacularTitle: 滑液间充质干细胞在无血清培养条件下生物学特性及体外三维软骨构建
- Author:
Jin NING
1
;
Zhaoyuan YUAN
;
Yu LIU
;
Yilin CAO
;
Shengjian TANG
;
Guangdong ZHOU
Author Information
1. Weifang Medical University, Weifang 261041
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Synovial fluid;
Culture media, serum-free
- From:
Chinese Journal of Plastic Surgery
2017;33(3):213-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the biological characteristics of synovial fluid-derived mesenchymal stem cells (SF-MSCs) cultured in serum-free medium and the ability of in vitro reconstruction of three-dimensional cartilage combined with scaffold material.
Methods:Human SF-MSCs were cultured in serum medium and mesenchymal stem cells medium-serum free (MSCM-sf) respectively, then the proliferative ability and morphology of SF-MSCs were compared; The third passage SF-MSCs cultured in MSCM-sf were identified by flow cytometry, three-way(chondrogenic, osteogenic, adipogenic)differentiation assay and induced for chondrogenic differentiation when combined with polyglycolic acid/polylactic acid (PGA/PLA).
Results:SF-MSCs cultured in MSCM-sf had better morphology and proliferative ability than that cultured in serum medium. The expression levels of positive markers of the third passage SF-MSCs cultured in MSCM-sf, such as CD73 (99.5%), CD90 (98.9%) and CD105 (96.5%), were more than 95%. However, the overall negative markers (CD34, HLA-DR and CD11b) expressed less than 2%. Three-way differentiation staining was positive. The combination of SF-MSCs and PGA / PLA can be induced into cartilage in vitro.
Conclusions:SF-MSCs cultured in MSCM-sf can be amplified under the condition of maintaining the stem cell characteristics, and can be combined with PGA/PLA scaffold to construct three-dimensional cartilage in vitro.
