Detection of HPV antibodies in the serum specimens of esophageal cancer patients in Chaoshan region
10.3760/cma.j.issn.1003-9279.2017.03.018
- VernacularTitle: 潮汕地区食管癌血清标本中HPV的抗体检测
- Author:
Si YE
1
;
Jintao LI
1
;
Zhenhai CAI
2
;
Yangjunqi WANG
1
;
Khodahemmati SARA
1
;
Weijian ZHAO
1
;
Hongxia LI
3
;
Yi ZENG
1
,
4
Author Information
1. Laboratory of Virology and Pharmocology, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
2. People′s Hospital of Jieyang City, Jieyang 522000, China
3. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Beijing 100052, China
4. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Beijing 100052, China
- Publication Type:Journal Article
- Keywords:
Esophageal neoplasms;
Papillomavirus human;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(3):262-265
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the presence of HPV associated antibodies in the serum of patients diagnosed with esophageal cancer in Chaoshan region and to provide data that could possibly be used as reference for the monitoring of patients with esophageal cancer.
Methods:The pSecTag recombinant vectors containing the HPV16 E6/E7 and HPV18 E6/E7 genes were constructed respectively to express HPV16 E6/E7 and HPV18 E6/E7 fusion proteins in 293 cell line after transfecting the cell line. Immunoenzymatic method was employed with the fusion proteins as antigen to detect IgG antibody against HPV in serum of 76 esophageal cancer patients and 149 normal persons undergoing health checkup. In addition, the same sera were assessed by enzyme-linked immunosorbent assay (ELISA) method using L1 as antigen.
Results:Only one esophageal cancer patient’s serum presented as positive for IgG by immunoenzyme technique when E6/E7 was used as antigen. However, when the L1 was used as antigen in ELISA assay, 37 of the 76 cases of esophageal cancer patients (48.7%) were HPV antibody-positive. Among the 149 health checkup persons, 79 (53.0%) were HPV antibody-positive. No significant difference was found between the two groups (P>0.05).
Conclusion:HPV related antibodies in the serum remain inapplicable as a screening tool for serological detection of esophageal cancer. Further research is needed to understand the role and diagnostic applications of HPV infection in esophageal cancer.
