Experimental study on the inhibitory effect of soap solution on rabies viruses
10.3760/cma.j.issn.1003-9279.2017.03.010
- VernacularTitle: 肥皂水对狂犬病病毒灭活作用的研究
- Author:
Shuqing LIU
1
;
Qian WANG
;
Yanrong LI
;
Xiaoyan TAO
;
Pengcheng YU
;
Xuexin LU
;
Weichen WU
;
Jianghong YAN
;
Wuyang ZHU
Author Information
1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory of Medical Virology, Ministry of Health, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Rabies virus;
Soap solutions;
Inhibitory effect
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(3):227-231
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inactivating effect of soap solution on rabies virus and to explore the concentration of soap solutions which could be effective in post-exposure prophylaxis (PEP) of rabies virus infection.
Methods:The BSR and N2a cells were respectively infected by the mixture of different concentrations of soap solution and rabies virus CVS-11. Based on the direct immunofluorescent method (DFA) and reverse transcription PCR (RT-PCR), the inactivating effects of soap solutions on rabies viruse in BSR and N2a cells were detected, respectively.
Results:This experiment established the BSR or N2a cells model in 24 well cell culture plates, and we found that the upper limit of soap solution concentration which BSR or N2a cells could tolerate was 1%. The inhibitory effect test of different soap solution on rabies virus showed that the 0.5% concentration of soap solution could completely inhibit the survival of CVS-11 strain in both the BSR and N2a cells, but the 0.1% concentration of soap solution could not inhibit the rabies viruses completely.
Conclusions:The 0.5%-1% concentration of soap solutions could inhibit the survival of CVS-11 strain in three minutes in vivo experiment.
