The influence of pVR-IL15 gene adjuvant on immunization with recombinant viruses expressing HBsAg
10.3760/cma.j.issn.1003-9279.2017.03.003
- VernacularTitle: pVR-IL15基因佐剂对表达HBsAg重组病毒免疫效果的影响
- Author:
Rui PAN
1
;
Bing HE
;
Guomin CHEN
;
Shengli BI
;
Yi ZENG
Author Information
1. State Key Laboratory for Infectious Disease Control and Prevention, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
pVR-IL15;
Gene adjuvant;
Recombinant virus
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(3):195-197
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the influence of pVR-IL15 gene adjuvant on the immune responses of different immunization strategies.
Methods:The sequential immunization strategies were used in BALB/c mice with a DNA vaccine, recombinant modified vaccinia virus Ankara and recombinant adenovirus expressing HBsAg respectively and combined with a gene adjuvant pVR-IL15. Cellular and humoral immune responses were evaluated by IFN-γ enzyme-linked immunospot assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA), respectively.
Results:The levels of humoral and cellular immune responses in the immune groups combined with pVR-IL15 were significantly higher than those of the non-combined with pVR-IL15.
Conclusions:The pVR-IL15 gene adjuvant can enhance the immune responses induced by the recombinant viruses expressing HBsAg.
