Determination of cadmium in human urine by colloidal gold immunochromatographic assay
10.3760/cma.j.issn.1001-9391.2017.03.018
- VernacularTitle: 人尿中镉离子测定的胶体金免疫层析快速检测法
- Author:
Guanyan FU
1
;
Jin GONG
;
Xiaoxiao LENG
Author Information
1. Occupational Disease Prevention and Control of Hospital in Chongqing, Chongqing 400060, China
- Publication Type:Journal Article
- Keywords:
Cadmium;
Gold colloid;
Immunochromatography;
Test paper strip
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(3):223-225
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the method of colloidal gold immunochrom atographic assay for detecting cadmium ions rapidly.
Methods:The anti-cadmium ion monoclonal antibody-gold conjugate was labeled on the binding pad, cadmium ion hapten and goat anti-mouse IgG were coated on nitrocellulose membrane as the detection line (T line) and quality control line (C line) respectively. The sample pad, colloidal gold bonding pad, nitrocellulose membrane and absorption pad were orderly assembled on the PVC board to cut into a test paper strip. The qualitative results of the assay were visualized in color.
Results:When detecting the human urine cadmium ions, the results were tested qualitativly within 15 minutes. The detection limit was 30 μg/L. No cross-reactivity with other heavy metal ions. The test paper strip could be stored at 4 ℃ for 3 months.
Conclusion:The method has the advantages of low cost, strong specificity, good stability and reliable results, and is suitable for rapid screening of cadmium poisoning of enterprise and occupational health.
