Determination of thiocyanate within human urine by ion chromatography method
10.3760/cma.j.issn.1001-9391.2017.03.017
- VernacularTitle: 人尿中硫氰酸根测定的离子色谱法
- Author:
Sheng WANG
1
;
Xingfu PAN
;
Wei ZHAO
;
Huifang YAN
Author Information
1. National Institute of Occupational Health and Poison Control, China CDC, Beijing 100050, China
- Publication Type:Journal Article
- Keywords:
Chromatography;
Thiocyanate;
Ions;
Urine
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(3):220-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish ion chromatography method to determine thiocyanate within urine of workers who were exposed to cyanide.
Methods:After the workers work, used 50 ml centrifuge tube to collect the urine of workers about 20 ml.The urine were tested by centrifugation, dilution and filtration by C18 column, thiocyanate was separated by AS16 and mobile phase elution by KOH, detected by electrical circuitryconductivity detector, quantitative by the standard curve method.
Results:The linear correlation coefficient of thiocyanate within the range of 0.1-5.0 μg/ml was more than 0.999. The method detection limit was 0.11μg/ml (in 1ml urine) , the method quantitative limit was 0.35 μg/ml. The method recoveries were 95.1%-99.7%. The within-day precision range was 0.54%-2.05%, The between-run precision range was 2.06%-5.09%. Sample stability test showed that thiocyanate samples could be stored for 5 days at room temperature and 7 days at 4 ℃, could be stored for 14 days at-20 ℃.
Conclusion:The technical indicator of method compliance with rule of Guide for establishing occupation health standards-Part 5: determination methods of chemicals in biological materials (GBZ/T 210.5-2008) , the method applies to workers who were exposed to cyanide.
