Influence of aluminum chloride exposure on embryonic development of zebrafish and neurobehavior of juvenile fish
10.3760/cma.j.issn.1001-9391.2017.03.002
- VernacularTitle: 氯化铝暴露对斑马鱼胚胎发育和幼鱼神经行为的影响
- Author:
Jianping CHEN
1
;
Nan SHANG
;
Kaihong HE
;
Ling ZHANG
;
Qiao NIU
;
Qinli ZHANG
Author Information
1. Department of Occupational Medicine, Shanxi Medical University, Taiyuan 030001, China
- Publication Type:Journal Article
- Keywords:
Aluminium chloride;
Zebrafish;
Embryos;
Developmental neurotoxicity
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(3):166-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of aluminum chloride (AlCl3) solution on the embryon-ic development of zebrafish and neurobehavior of juvenile fish.
Methods:The embryos of zebrafishat 6 hours after fertilization were exposed to AlCl3 solution at a concentration of 0, 55.0, 60.5, 66.6, 73.5, 80.5, or 100.0 mg/L, and embryonic hatching rates at 48 and 72 hours after fertilization were calculated. The embryos of zebrafishat 6 hours after fertilization were exposed to AlCl3 solution at a concentration of 0, 60.0, 72.0, 86.4, 103.7, or 124.4 mg/L, and the embryonic mortality rates at 12, 24, 48, 72, and 96 hours after fertilization were calculat-ed. The embryos of zebrafish at 6 hours after fertilization were exposed to AlCl3 solution at a concentration of 0, 50, 100, 200, 400, or 800 μg/L, and the changes in the neurobehavior of juvenile fish were observed after hatching, including touch-escape reaction at 72 hours after fertilization and autonomic movement and panic es-cape reflex at 7 days after fertilization.
Results:Compared with the 0 mg/L group, the≥66.6 mg/L group had a sig-nificant reduction in embryonic hatching rate at 48 and 72 hours after fertilization, and the ≥72.0 mg/L group had a significant increase in embryonic mortality rate at 96 hours after fertilization (P<0.05) . Compared with the 0 μg/L group, the≥100 μg/L group had a significant reduction in the number of times of touch-escape reaction (P<0.05) .Compared with the 0 and 50 μg/L groups, the 100-800 μg/L groups had significant reductions in total movement distance and average speed (P<0.05) . Compared with the dark period before illumination, all groups had a significant increase in movement speed during the light period of the panic escape reflex test (i.e., the third minute) (P<0.05) ; within 2 minutes after the light was turned off, there was no significant change in movement speed in the 0-200 μg/L groups (P>0.05) ; the 400 and 800 μg/L groups had a significant increase in movement speed (P<0.05) .
Conclusion:AlCl3 exposure may cause embryonic developmental disorder in zebrafish and ab-normal neurobehavior in juvenile fish.
