The composition of the gastrointestinal bacterial flora of mouse embryos and the placenta tissue bacterial flora
10.3760/cma.j.issn.0578-1310.2017.03.007
- VernacularTitle: 小鼠胚胎胃肠道和胎盘组织的菌群构成研究
- Author:
Da LEI
1
;
Yan LIN
;
Xun JIANG
;
Li LAN
;
Wei ZHANG
;
Baoxi WANG
Author Information
1. Department of Pediatrics, Tangdu Hospital, the Fourth Military Medical University, Xi′an 710038, China
- Publication Type:Journal Article
- Keywords:
Bacteria;
Mice;
Gastrointestinal tract;
Placenta;
High-throughput nucleotide sequencing
- From:
Chinese Journal of Pediatrics
2017;55(3):200-204
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the composition of the gastrointestinal bacterial flora of mouse embryos and the placenta tissue bacterial flora.
Method:Twenty-four specimens were collected from pregnant Kunming mouse including 8 mice of early embryonic (12-13 days) gastrointestinal tissues, 8 cases of late embryonic (19-20 days)gastrointestinal tissues, 8 of late pregnancy placental tissues.The 24 samples were extracted by DNeasy Blood & Tissue kit for high-throughput DNA sequencing.
Result:The level of Proteobacteria, Bacteroidetes, Actino-bacteria and Firmicutes were predominantin all specimens.The relative content of predominant bacterial phyla in each group: Proteobacteria (95.00%, 88.14%, 87.26%), Bacteroidetes(1.71%, 2.15%, 2.63%), Actino-Bacteria(1.16%, 4.10%, 3.38%), Firmicutes(0.75%, 2.62%, 2.01%). At the level of family, there were nine predominant bacterial families in which Enterobacteriaeae, Shewanel laceae and Moraxellaceae were dominant.The relative content of dominant bacterial family in eachgroup: Enterobacteriaeae (46.99%, 44.34%, 41.08%), Shewanellaceae (21.99%, 21.10%, 19.05%), Moraxellaceae(9.18%, 7.09%, 5.64%). From the species of flora, the flora from fetal gastrointestinal in early pregnancy and late pregnancy (65.44% and 62.73%) were the same as that from placenta tissue in the late pregnancy.From the abundance of bacteria, at the level of family, the same content of bacteria in three groups accounted for 78.16%, 72.53% and 65.78% respectively.
Conclusion:It was proved that the gastrointestinal bacterial flora of mouse embryos and the placenta tissue bacterial flora were colonized. At the same time the bacteria are classified.
