Expression of Septin2 in Hodgkin lymphoma cell line L428 and its role in promoting H/RS cells’ redifferentiation to B lymphocytes
10.3760/cma.j.issn.0253-2727.2017.02.010
- VernacularTitle: Septin2在霍奇金淋巴瘤细胞株L428细胞中的表达及其在促进细胞再分化中的作用
- Author:
Qingcan SUN
1
;
Lin ZHONG
;
Bo QIU
;
Tong ZHAO
;
Xinhua ZHOU
1
Author Information
1. Department of Pathology, Southern Medical University, Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, China
- Publication Type:Journal Article
- Keywords:
Hodgkin disease;
Septins;
L428 cells;
Reed-Sternberg cells;
Cell differentiation
- From:
Chinese Journal of Hematology
2017;38(2):134-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of GTP binding protein 2 (Septin2) in the differentiation of Hodgkin’s Lymphoma H/RS cells (Hodgkin/Reed-Sternberg) to B lymphocytes.
Methods:The expressions of Septin2 mRNA and protein in Hodgkin’s Lymphoma cell line L428 which CD99 was overexpressed were detected by RT-qPCR and Western blot,confocal laser microscopy and immunocytochemistry (ICC) . RT-qPCR and Western blot were used to assay the expression of Septin2 after Septin2-siRNA transfected into L428 cells, and confocal laser microscopy, CCK8 assay and flow cytometry were used to analyze the changes of F-actin cytoskeleton,cell proliferation ability and immunophenotype.
Results:The low expressions of Septin2 mRNA and protein were detected in L428 cell line after overexpresion of CD99 (0.329±0.019 vs 1.000, P=0.001) and Septin2 siRNA transfection (0.276±0.025 vs 1.000, P=0.000) compared to controls. Compared to vector group, Septin2 siRNA transfection could lead to decrease of cell size, decline of proliferation activity (F=204.927, P<0.001) and reconstruction of F-actin cytoskeleton, and the expression of specific antigen markers CD30 and CD15 of H/RS cell decreased, B cell antigen marker CD19 as well as germinal center marker CD10 and early plasma cell marker CD38 were up-regulated.
Conclusion:Septin2 interference promotes H/RS cells’ redifferentiation to B lymphocytes
