Comparisons for kits HAVAb2.0 and HAV-IgG
10.3760/cma.j.issn.1003-9279.2017.02.019
- VernacularTitle: Abbott公司HAVAb2.0和HAV-IgG试剂的评价
- Author:
Wenting ZHOU
1
;
Feng WANG
1
;
Changhong HUANG
2
;
Shoujun ZHAO
3
;
Yong ZHANG
1
;
Shengli BI
1
;
Jingyuan CAO
1
Author Information
1. Key Laboratory for Medical Virology, Minstry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 10026, China
2. Kaifeng center for disease control and prevention, Henan province, Kaifeng 475000, China
3. Department of Radiology, University of California, San Francisco 94143, USA
- Publication Type:Journal Article
- Keywords:
Antibodies to hepatitis A virus;
Diagnostic test;
Reliability;
Validity
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(2):169-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Two kits for detecting anti-HAV antibody produced by Abbott Laboratories are evaluated for their performance in order to use in the anti-HAV antibodies detections.
Methods:Serially diluted standard reference serum were detected 4 times with HAVAb2.0 kit and HAV-IgG kit , then fit standard curves and calculated Interclass correlation coefficient (ICC). 120 serum that have different levels of anti-HAV antibodies were chosen to be detected by two kits and calculated sensitivity and specificity, receive operation characteristic (ROC) curve and area under curve (AUC) while the results of HAV-IgG were used as golden standard.
Results:R2 and ICC for HAV-IgG were 0.9977 and 0.999, respectively, higher than 0.9893 and 0.995(P>0.05) for HAVAb2.0. Sensitivity and specificity for HAVAb2.0 were 93.15% and 100% and AUC was 0.994 when kit HAV-IgG was chosen as golden standard.
Conclusion:There is little difference in performance between HAV-IgG and HAVAb2.0, both of them can be used in anti-HAV antibodies detection.
