Rapid construction of stable cell lines which inducible express HA proteins from various influenza virus strains
10.3760/cma.j.issn.1003-9279.2017.02.016
- VernacularTitle: 可诱导性表达多种流感病毒HA蛋白稳定细胞系新型构建方法的研究
- Author:
Yanjing ZHANG
1
;
Lianlian HAN
;
Li GUO
;
Hui ZHONG
;
Jianwei WANG
Author Information
1. Peking Union Medical College & Institute of Pathogen Biology Chinese Academy of Medical Sciences, Beijing 100730, China
- Publication Type:Journal Article
- Keywords:
Influenza virus;
PiggyBac transposon;
Inducible expression;
Cell line construction
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(2):157-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective:An innovative technique was established to rapidly construct various cell lines that could be induced to express multiple influenza A virus (IAV) proteins.
Method:The HA protein genes of multiple IAVs were cloned into the Cumate-induced expression system which was positioned between two PiggyBac transposon sites. These HA plasmids were transfected into the HEK293A cell line with the PiggyBac transposase plasmids. The transfected cells were screened with puromycin, and after that the corresponding virus proteins were induced with Cumate.
Results:The results of flow cytometry and Western blotting showed that the virus proteins were expressed in most of the cells in corresponding lines after the induction of Cumate.
Conclusion:Cell lines which were inducible to express IVA HA protein can be efficiently constructed by using the PiggyBac transposon system.
