Molecular epidemiology of Norovirus infection among sporadic hospitalized adults with diarrhea in Qingdao, 2015
10.3760/cma.j.issn.1003-9279.2017.02.004
- VernacularTitle: 青岛市2015年成人散发腹泻病例诺如病毒感染分子流行病学特征
- Author:
Dan ZHAO
1
;
Zhilei SU
;
Feng ZHANG
;
Xiaoyan SHI
;
Zhaoguo WANG
Author Information
1. Qingdao Municipal Center for Disease Control and Prevention, Qingdao 266033, China
- Publication Type:Journal Article
- Keywords:
Norovirus;
Diarrhea;
Genotype;
Molecular epidemiology
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(2):104-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the molecular epidemiology of Norovirus(NoV) infection among sporadic hospitalized adults with diarrhea in Qingdao, 2015.
Methods:Four hundred and nine stool samples were collected from hospitalized adults with diarrhea and detected by Real-time RT-PCR. For genotyping, ORF1 and ORF2 were partially amplified by RT-PCR and sequenced.
Results:18.1%(74/409) of stool samples were positive for NoV genogroup I(GI) (10/74) and genogroup II(G II) (64/74). Fifty-three positive samples of GII were sequenced and divided into 4 genotypes, including GII.Pe-GII.4(26/53), GII.P17-GII.17(19/53), GII.P12-GII.3(7/53) and GII.P16-GII.13(1/53). All GII.4 strains were the variants of GII.4-Sydney-2012. From May to Aug in 2015, GII.4 only accounted for 5.3%(1/19) and GII.17 were the major epidemic strains(68.4%, 13/19). But from Sept to Dec in 2015, GII.17 decreased substantially to 10.9%(6/55) and GII.4 became the most predominated strains (45.5%, 25/55).
Conclusions:NoV is an important pathogen responsible for viral diarrhea among adults in Qingdao. GII.4-Sydney-2012 and the newly variant GII.P17-GII.17 were the predominant epidemic strains in Qingdao, 2015.
